Periostin antibody (C-Term)

Catalog No. ABIN6657608

Supplier Product No.: 600-401-e09

This anti-Periostin antibody is a Rabbit Polyclonal antibody detecting Periostin in WB and IHC. Suitable for Mouse.

Quick Overview for Periostin antibody (C-Term) (ABIN6657608)

Target: Periostin (POSTN)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Periostin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Periostin Antibody

Binding Specificity: C-Term

Supplier Product No.: 600-401-e09

Supplier: Rockland

Purpose: Periostin Antibody

Cross-Reactivity (Details): Anti-Periostin antibody is directed against mouse Periostin protein.

Purification: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: Anti-Periostin Antibody was produced by repeated immunizations with bacterial fusion proteins equivalent to a 188 amino acid polypeptide from the C-terminal region of mouse periostin which is comprised of six small alternatively spliced exons.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 1:100

Western_Blot_Dilution: 1:1000

Other: User Optimized

Comment:

Anti-Periostin Antibody is tested for use in Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 93 kDa in size corresponding to periostin doublet in mouse lung extract. The antibody works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90° C in 10 mM sodium citrate (pH 6.0)/ 0.1 % Tween-20).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Periostin

Target: Periostin (POSTN)

Alternative Name: Periostin

Background:

Synonyms: PN, Osteoblast-specific factor 2, OSF-2

Background: Periostin Antibody detects Periostin protein. Periostin is a matricellular protein, i.e. an extracellular matrix protein that interacts both with other ECM proteins and with cell-surface receptors. Like many other matricellular proteins, the function of periostin is important both in embryonic development and in the remodeling of adult tissues in response to pathological insults. Periostin was originally isolated as an osteoblast-specific marker that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Periostin has since been demonstrated to be important in heart valve development and myocardial infarction because it promotes collagen fibrogenesis, inhibits differentiation of progenitor cells into cardiomyocytes and is essential in maintaining the biomechanical properties of the adult myocardium. Anti-Periostin Antibody is ideal for investigators involved in Cancer and Cardiovascular research.

Gene Name: POSTN

Gene ID: 50706

UniProt: Q62009