Ataxin 1 antibody (AA 746-761) (PE)

Catalog No. ABIN6657681

Supplier Product No.: 200-308-w87

This Mouse Monoclonal antibody specifically detects Ataxin 1 in WB, IHC and ELISA. It exhibits reactivity toward Mouse.

Quick Overview for Ataxin 1 antibody (AA 746-761) (PE) (ABIN6657681)

Target: Ataxin 1 (ATXN1)

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ataxin 1 antibody is conjugated to PE

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: S65-37

Product Details anti-Ataxin 1 Antibody (PE)

Binding Specificity: AA 746-761

Supplier Product No.: 200-308-w87

Supplier: Rockland

Purpose: Ataxin 1 Antibody Phycoerythrin

Cross-Reactivity (Details): BLAST analysis suggests that it is 93 % identical to rat and 87 % identical to human.

Purification: Anti-Ataxin 1 Antibody was purified from concentrated tissue culture supernate by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: Anti-Ataxin 1 Antibody was produced by repeated immunization of mice with a synthetic peptide containing amino acids 746-761 of mouse ataxin-1.

Isotype: IgG1

Application Details

Application Notes:

ELISA_Dilution: 1:10,000

Western_Blot_Dilution: 1:1000

Comment:

Anti-Ataxin 1 R-PE Conjugated Antibody is suitable for Western Blots and Immunocytochemistry. Expect a band approximately ~85kDa on specific lysates or tissues. No cross reactivity against phosphor S751-Ataxin-1. Minimal cross reactivity against S751A mutant of Ataxin-1 by ELISA and immunofluorescence and negative by immunoblot. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.1 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Expiry Date: 12 months

Target Details for Ataxin 1

Target: Ataxin 1 (ATXN1)

Alternative Name: Ataxin 1

Background:

Synonyms: Ataxin-1, ATX1, Atxn1, D6S504E, OTTHUMP00000016065, SCA1, Spinocerebellar ataxia type 1 protein, Spinocerebellar ataxia type 1 protein homolog

Background: Ataxin-1 is a member of the ATXN1 protein family and contains a single AXH domain. It is a neurodegenerative disorder protein thought to have a role in the metabolism of RNA as it has been shown to localize to the RNA and transcription dependent inclusions within the nucleus. A mutation of Ataxin-1 is the cause of spinocerebellar ataxia type-1 (SCA1), a progressive, neurodegenerative disease that is autosomal dominant and primarily affects the Purjinke cells found in brain stem neuronal populations and the cerebellum. Expression of Ataxin-1 is almost ubiquitous, except in the brain where it is isolated to populations of neurons. Anti-Ataxin-1 is ideal for research in Neuroscience.

Gene Name: Ataxin-1

Gene ID: 20238

UniProt: P54254

Pathways: Synaptic Membrane