BAT3 antibody (C-Term)

Catalog No. ABIN6657746

Supplier Product No.: 100-401-b63

This anti-BAT3 antibody is a Rabbit Polyclonal antibody detecting BAT3 in WB and ELISA. Suitable for Mouse. This Primary Antibody has been cited in 2+ publications.

Quick Overview for BAT3 antibody (C-Term) (ABIN6657746)

Target: BAT3 (Large Proline-Rich Protein BAT3 (BAT3))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAT3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-BAT3 Antibody

Binding Specificity: C-Term

Supplier Product No.: 100-401-b63

Supplier: Rockland

Purpose: Scythe/Bat3 Antibody

Purification: This product was prepared from monospecific antiserum by addition of Sodium azide to 0.01 % .

Sterility: Sterile filtered

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal portion of mouse Scythe/BAT3.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: User Optimized

Western_Blot_Dilution: 1:5,000 to 1:8000

Other: User Optimized

Comment:

This polyclonal antibody has been tested for use in western blotting and is specific for mouse Scythe/Bat3 and its isoforms. Specific conditions for reactivity should be optimized by the end user. Expect multiple bands, with the predominant at approximately 200kDa in size corresponding to Scythe/BAT3 by western blotting in the appropriate cell lysate or extract. In western blot analysis of endogenous protein, multiple bands of Scythe/BAT3 may appear corresponding to variable amount of splice isoforms present.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-BAT3 antibody (ABIN6657746)

Desmots, Russell, Michel, McKinnon: "Scythe regulates apoptosis-inducing factor stability during endoplasmic reticulum stress-induced apoptosis." in: The Journal of biological chemistry, Vol. 283, Issue 6, pp. 3264-71, (2008) (PubMed).

Desmots, Russell, Lee, Boyd, McKinnon: "The reaper-binding protein scythe modulates apoptosis and proliferation during mammalian development." in: Molecular and cellular biology, Vol. 25, Issue 23, pp. 10329-37, (2005) (PubMed).

Target Details for BAT3

Target: BAT3 (Large Proline-Rich Protein BAT3 (BAT3))

Alternative Name: Scythe/Bat3

Background:

Synonyms: rabbit anti-Bat3 Antibody, rabbit anti-Scythe Antibody, Human Leukocyte Antigen-B-Associated Transcript 3, HLA-B-associated transcript 3, BAT3, Large proline-rich protein BAT3

Background: Scythe protein (also known as BAT3 and HLA-B-associated transcript 3) is an apoptotic regulator that is highly conserved in eukaryotes and contains a ubiquitin-like domain near its N-terminus. It binds Reaper, a potent apoptotic inducer, as well as Hid and Grim. Scythe and Reaper are thought to signal apoptosis, in part through regulating the folding and activity of apoptotic signaling molecules. Scythe regulates apoptosis-inducing factor stability during endoplasmic reticulum stress-induced apoptosis. It interacts with transforming growth factor-beta (TGF-beta) receptors and enhances TGF-beta1-induced type I collagen expression in mesangial cells. With SET1A, Scythe forms a complex with CTCFL/BORIS to modulate H3K4 histone dimethylation and gene expression. Scythe is essential for p300-mediated acetylation of p53. Scythe/BAT3 is identified as a critical regulator of Hsp70-2 in spermatogenesis, thereby providing a possible molecular target in idiopathic male infertility. The human Scythe/BAT3 ortholog in rodents is predominantly and developmentally expressed in testis.

Gene Name: BAT3

Gene ID: 224727, 33147082

UniProt: Q9Z1R2