CD69 antibody (PE)

Catalog No. ABIN6657782

Supplier Product No.: 200-b08-n88

This anti-CD69 antibody is a Hamster Monoclonal antibody detecting CD69 in FACS, IHC and IP. Suitable for Mouse.

Quick Overview for CD69 antibody (PE) (ABIN6657782)

Target: CD69

Reactivity: Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This CD69 antibody is conjugated to PE

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Clone: H1-2F3

Product Details anti-CD69 Antibody (PE)

Supplier Product No.: 200-b08-n88

Supplier: Rockland

Purpose: CD69 Phycoerythrin Antibody

Cross-Reactivity (Details): Cross reactivity with CD69 from other sources has not been tested.

Purification: Phycoerythrin conjugated CD69 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD69.

Sterility: Sterile filtered

Immunogen: Anti-CD69 Antibody (Monoclonal) was produced by repeated immunizations with Mouse dendritic epidermal T cell line Y245.

Isotype: IgG

Labeling Ratio: 1-2

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)

Comment:

Anti-CD69 is tested for FLOW and useful for Immunohistochemistry and Immunoprecipitation using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Expiry Date: 6 months

Target Details for CD69

Target: CD69

Alternative Name: CD69

Background:

Synonyms: Early activation antigen CD69, CD69, Cd69

Background: CD69 is a 60 kD type II membrane protein composed of a 27/33 kD disulfide-linked homodimer, also known as Very Early Activation Antigen (VEA), AIM, EA1, MLR3, and gp34/28. It is expressed on a subset of thymocytes and platelets. CD69 is rapidly induced on activated T and B cells, neutrophils, and NK cells. It is a C-type lectin, closely related to the NKR-P1 and Ly-49 NK cell activation molecules. CD69 is involved in the early events of cell activation and thymocyte positive selection.

Gene Name: Cd69

Gene ID: 12515

NCBI Accession: NP_001028294

UniProt: P37217