COX IV antibody (pSer58)

Catalog No. ABIN6657951

Supplier Product No.: 600-401-x00

This Rabbit Polyclonal antibody specifically detects COX IV in WB and ELISA. It exhibits reactivity toward Mouse.

Quick Overview for COX IV antibody (pSer58) (ABIN6657951)

Target: COX IV (COX4I1) (Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COX IV antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-COX IV Antibody

Binding Specificity: pSer58

Supplier Product No.: 600-401-x00

Supplier: Rockland

Purpose: CoxIV phospho S58 Antibody

Purification: Anti-Phospho-Ser58 COXIV Antibody was prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Sterility: Sterile filtered

Immunogen: Anti-Phospho-Ser58 COXIV Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser58 of mouse COXIV protein conjugated to KLH.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000

Western_Blot_Dilution: 1:1000

Comment:

Anti-Phospho-Ser58 COXIV Antibody has been tested by Western Blots and is specific for the ~17k COXIV-1 protein phosphorylated at Ser58. Immunolabeling is blocked by the phospho-peptide used as antigen but not by the corresponding dephospho-peptide. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Preservative: None

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for COX IV

Target: COX IV (COX4I1) (Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1))

Alternative Name: COXIV

Background:

Synonyms: Cytochrome c oxidase subunit 4 isoform 1 mitochondrial, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit IV isoform 1, COX IV-1

Background: COX, also known as cytochrome c oxidase, has 12 subunits that make up the transmembrane mitochondrial protein. Subunit IV has two isoforms, COXIV-1 and COXIV-2. COXIV-1 is expressed ubiquitously while COXIV-2 is highly expressed in adult lung and low levels in brain and heart. Phosphorylation of amino acid residue ser58 of the COXIV-1 protein is a PKA-dependent regulation of COX and plays an important role in metabolism and CREB cycle activation. COX IV pS58 Antibody is ideal for researchers interested in cell cycle research.

Gene Name: COX4i1

Gene ID: 12857

NCBI Accession: NP_034071

UniProt: P19783

Pathways: Proton Transport