SHANK2 antibody (Internal Region)

Catalog No. ABIN6658069

Supplier Product No.: 200-301-g42

The Mouse Monoclonal anti-SHANK2 antibody has been validated for WB, IHC, IP, IF and FM. It is suitable to detect SHANK2 in samples from Rat.

Quick Overview for SHANK2 antibody (Internal Region) (ABIN6658069)

Target: SHANK2 (SH3 and Multiple Ankyrin Repeat Domains 2 (SHANK2))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SHANK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Clone: N23b-49

Product Details anti-SHANK2 Antibody

Binding Specificity: Internal Region

Supplier Product No.: 200-301-g42

Supplier: Rockland

Purpose: PAN-SHANK Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with SHANK from human, mouse, and rat based on 100 % homology with the immunizing sequence.

Purification: Anti-PAN-SHANK Antibody was purified by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: SHANK Antibody was produced in mice by repeated immunizations raised against a fusion protein corresponding to an internal region of rat Shank2.

Isotype: IgG1

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: 0.1-1.0 μg/mL

IF_Microscopy_Dilution: 1.0-10 μg/mL

Western_Blot_Dilution: 1-10 μg/mL

Comment:

Anti-SHANK Antibody is tested for use in WB, IHC and IP. Expect a band approximately ~160kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for SHANK2

Target: SHANK2 (SH3 and Multiple Ankyrin Repeat Domains 2 (SHANK2))

Alternative Name: Shank2

Background:

Synonyms: SH3 and multiple ankyrin repeat domains protein 2, Shank2, Cortactin-binding protein 1, CortBP1, GKAP/SAPAP-interacting protein, Proline-rich synapse-associated protein 1, ProSAP1, SPANK-3

Background: Shank proteins make up a family of scaffold proteins identified through their interaction with a variety of membrane and cytoplasmic proteins. Shank proteins at postsynaptic sites of excitatory synapses play roles in signal transmission into the postsynaptic neuron.

Gene Name: Shank2

Gene ID: 171093

NCBI Accession: NP_958738

UniProt: Q9QX74