MARCKS antibody (pSer152, pSer156)

Catalog No. ABIN6658212

Supplier Product No.: 612-401-d70

This Rabbit Polyclonal antibody specifically detects MARCKS in WB. It exhibits reactivity toward Rat.

Quick Overview for MARCKS antibody (pSer152, pSer156) (ABIN6658212)

Target: MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MARCKS antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MARCKS Antibody

Binding Specificity: pSer152, pSer156

Supplier Product No.: 612-401-d70

Supplier: Rockland

Purpose: MARCKS phospho S152/phospho S156 Antibody

Cross-Reactivity (Details): MARCKS antibody are affinity purified from monospecific antiserum by immunoaffinity purification.

Purification: Anti-MARCKS pS152/pS156 antibody is directed against MARKS doubly phosphorylated at Ser152 and Ser156.

Immunogen: Anti-MARCKS pS152/pS156 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser 152/156 conjugated to KLH.

Isotype: IgG

Application Details

Application Notes: Optional[Neutralization_Dilution]: 1:1000

Comment:

Anti-MARCKS pS152/pS156 (Rabbit) antibody is tested for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 87 kDa in size corresponding to MARCKS protein phosphorylated at Ser 152/156 in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for MARCKS

Target: MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))

Alternative Name: MARCKS

Background:

Synonyms: Myristoylated alanine-rich C-kinase substrate, Protein kinase C substrate 80 kDa protein

Background: MARCKS pS152/pS156 antibody detects MARKS when phosphoylated at both Ser152 and Ser156 Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a major substrate for phosphorylation by protein kinase C (PKC). The phosphorylation of Ser152/156 can be used as a measure of PKC activation although these sites are also phosphorylated by PRK1 MARCKS is a member of a family of calmodulin binding proteins and phosphorylation of Ser 152/156 modulates the binding of MARCKS to calmodulin. Therefore, phosphorylated MARCKS antibody is ideal for investigators involved in Cell Signaling, Cell migration and Cancer Research.

Gene Name: MARCKS

Gene ID: 3028

UniProt: P30009