Synaptojanin 1 antibody (C-Term)

Catalog No. ABIN6658254

Supplier Product No.: 212-301-e35

Product Details anti-Synaptojanin 1 Antibody

Target: Synaptojanin 1 (SYNJ1)

Binding Specificity: C-Term

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Synaptojanin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Supplier Product No.: 212-301-e35

Supplier: Rockland

Purpose: Synaptojanin 1 Antibody

Cross-Reactivity (Details): Anti-Synaptojanin 1 Monoclonal Antibody is directed against rat synaptojanin 1

Purification: .Synaptojanin 1 antibody was Protein G purified from cell culture supernatant.

Immunogen: Anti-Synaptojanin 1 Monoclonal Antibody was produced in mouse by repeated immunizations with recombinant protein from the C-terminal region of rat synaptojanin 1.

Clone: 5H1

Isotype: IgG1

Application Details

Application Notes:

Immunohistochemistry_Dilution: 1:1000

Western_Blot_Dilution: 1:1000

Comment:

Anti-Synaptojanin 1 antibody is tested for use in Western Blotting and IHC. Expect a band of approximately 145 kDa in size corresponding to synaptojanin I proteins of rat brain extracts. Researchers should determine optimal titers for applications that are not stated below.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Synaptojanin 1

Target: Synaptojanin 1 (SYNJ1)

Alternative Name: Synaptojanin 1 (SYNJ1 Products)

Background:

Synonyms: mouse anti-Synaptojanin 1 Antibody, Synaptic inositol-1,4,5-trisphosphate 5-phosphatase 1

Background: Synaptojanin 1 Monoclonal Antibody detects Synaptojanin 1, which is a phosphatidylinositol phosphatase involved in clathrin-mediated endocytosis of synaptic vesicles. Synaptojanin 1 has two alternatively spliced isoforms, one that is ~ 145 kDA and is exclusively expressed in neurons, and the other ~ 170 kDa which is expressed in non-neuronal, peripheral tissues. The gene which encodes Synaptojanin 1, SYNJ1, has been mapped to chromosome 21 thus making it a candidate for involvement in Down's syndrome (DS). It has recently been demonstrated that Ts65Dn mice (the most commonly used model of DS) have altered phosphatidylinositol-4,5-bisphosphate metabolism. This defect is rescued by restoring SYNJ1 to disomy in the Ts65Dn mice.

Gene Name: SYNJ1

Gene ID: 85238

UniProt: Q62910

Pathways: Inositol Metabolic Process, Synaptic Vesicle Exocytosis