Interferon gamma antibody (AA 75-155)

Catalog No. ABIN669126

This anti-Interferon gamma antibody is a Rabbit Polyclonal antibody detecting Interferon gamma in WB, ELISA, FACS, IHC (p), IHC (fro), IF (p) and IF (cc). Suitable for Mouse and Rat. This Primary Antibody has been cited in 21+ publications.

Quick Overview for Interferon gamma antibody (AA 75-155) (ABIN669126)

Key Features:

• High quality Interferon gamma Primary Antibody for the detection of IFNG.
• Reliable product with high standard validation data

Target: Interferon gamma (IFNG)

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Interferon gamma antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Product Details anti-Interferon gamma Antibody

Binding Specificity: AA 75-155

Specificity: This antibody has been shown to work in frozen tissue section IHC as well, but is not guaranteed for this application.

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse IFN gamma

Isotype: IgG

Product Specific Information:

What can the Interferon gamma Antibody ABIN669126 be used for?: This polyclonal Interferon gamma Antibody detects Interferon gamma. The Interferon gamma Antibody has been validated for various applications and can be used for the detection of Interferon gamma and derivatives by Western Blotting, ELISA, Flow Cytometry, Immunohistochemistry (Paraffin-embedded Sections), Immunohistochemistry (Frozen Sections), Immunofluorescence (Paraffin-embedded Sections), Immunofluorescence (Cultured Cells).

What validation data is available for this Interferon gamma Antibody?: The primary antibody is referenced in 20 publications and is characterized by a proven, very high reliability. It has currently 7 product images that show its performance in a variety of applications. The product is currently available in 100 μL quantity. Interferon gamma Antibody for the detection of Interferon gamma and derivatives.

What is the function of Interferon gamma?: This gene encodes a member of the type II interferon family. The protein encoded is a soluble cytokine with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. Mutations in this gene are associated with aplastic anemia.[provided by RefSeq, Nov 2009].

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Interferon gamma antibody (ABIN669126)

Penna-Martinez, Kammerer, Stützle, Fees, Behr, Schaible, Schröder, Verboket, Neijhoft, Marzi, Nau, Henrich: "Enhancement of a one-step membrane technique for the treatment of large bone defects by pre-seeding the membrane with CD8 lymphocyte depleted bone marrow mononuclear cells in a rat femoral defect model." in: Frontiers in immunology, Vol. 15, pp. 1488611, (2024) (PubMed).

Wang, Tang, Shen, Chen, Chen, Luo, Ge: "Hepatocyte growth factor (HGF) optimizes oral traumatic ulcer healing of mice by reducing inflammation." in: Cytokine, Vol. 99, pp. 275-280, (2018) (PubMed).

Tuorkey: "Effects of Moringa oleifera aqueous leaf extract in alloxan induced diabetic mice." in: Interventional medicine & applied science, Vol. 8, Issue 3, pp. 109-117, (2017) (PubMed).

Chuang, Jan, Chang, Lu: "The Chinese medicine JC-001 enhances the chemosensitivity of Lewis lung tumors to cisplatin by modulating the immune response." in: BMC complementary and alternative medicine, Vol. 17, Issue 1, pp. 210, (2017) (PubMed).

Hayashi, Kawakubo-Yasukochi, Mizokami, Hazekawa, Yakura, Naito, Takeuchi, Nakamura, Hirata: "Uncarboxylated Osteocalcin Induces Antitumor Immunity against Mouse Melanoma Cell Growth." in: Journal of Cancer, Vol. 8, Issue 13, pp. 2478-2486, (2017) (PubMed).

Venkatasubramanian, Tripathi, Tucker, Paidipally, Cheekatla, Welch, Raghunath, Jeffers, Tvinnereim, Schechter, Andrade, Mackman, Idell, Vankayalapati: "Tissue factor expression by myeloid cells contributes to protective immune response against Mycobacterium tuberculosis infection." in: European journal of immunology, Vol. 46, Issue 2, pp. 464-79, (2016) (PubMed).

Li, Honda, Arima, Yasui, Inami, Nishiura, Hashimoto, Matsumoto: "Interferon-γ enhances the efficacy of autogenous bone grafts by inhibiting postoperative bone resorption in rat calvarial defects." in: Journal of prosthodontic research, Vol. 60, Issue 3, pp. 167-76, (2016) (PubMed).

Cao, Wang, Du, Liu, Zhang, Feng, Jin: "l-arginine and docetaxel synergistically enhance anti-tumor immunity by modifying the immune status of tumor-bearing mice." in: International immunopharmacology, Vol. 35, pp. 7-14, (2016) (PubMed).

Kim, Park, Back, Park, Kim, Park, Yeo, Park: "Otitis media with effusion in an allergic animal model: A functional and morphological study." in: International journal of pediatric otorhinolaryngology, Vol. 84, pp. 6-11, (2016) (PubMed).

Makar, Nimmagadda, Singh, Lam, Mubariz, Judge, Trisler, Bever: "TrkB agonist, 7,8-dihydroxyflavone, reduces the clinical and pathological severity of a murine model of multiple sclerosis." in: Journal of neuroimmunology, Vol. 292, pp. 9-20, (2016) (PubMed).

Zhou, Li, Gao, Wang, Wang, Yan, Xie, Lv: "Sulindac has strong antifibrotic effects by suppressing STAT3-related miR-21." in: Journal of cellular and molecular medicine, Vol. 19, Issue 5, pp. 1103-13, (2015) (PubMed).

Gong, Hu, Liu, Han, Guo, Luo, Wang, Li, Huang: "Effects of Foxp3 gene modified dendritic cells on mouse corneal allograft rejection." in: International journal of clinical and experimental medicine, Vol. 8, Issue 3, pp. 3965-73, (2015) (PubMed).

Li, Hashimoto, Honda, Arima, Matsumoto: "The Effect of Interferon-γ and Zoledronate Treatment on Alpha-Tricalcium Phosphate/Collagen Sponge-Mediated Bone-Tissue Engineering." in: International journal of molecular sciences, Vol. 16, Issue 10, pp. 25678-90, (2015) (PubMed).

Zhang, Hu, Liu, Ye, Gui, Zhou, Qi, He, Wang, Wang: "CD11b deficiency suppresses intestinal tumor growth by reducing myeloid cell recruitment." in: Scientific reports, Vol. 5, pp. 15948, (2015) (PubMed).

Makar, Gerzanich, Nimmagadda, Jain, Lam, Mubariz, Trisler, Ivanova, Woo, Kwon, Bryan, Bever, Simard: "Silencing of Abcc8 or inhibition of newly upregulated Sur1-Trpm4 reduce inflammation and disease progression in experimental autoimmune encephalomyelitis." in: Journal of neuroinflammation, Vol. 12, pp. 210, (2015) (PubMed).

Lu, Liu, Gu, Ge, Wu, Ze-Ji, Qiu, Zhang, Chao, Yu: "Gene Expression Profiles Underlying Selective T-Cell-Mediated Immunity Activity of a Chinese Medicine Granule on Mice Infected with Influenza Virus H1N1." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2014, pp. 976364, (2014) (PubMed).

Wang, Hu, Wang, Gu, Gao, Cao, Zheng: "5-Aminolevulinic acid-mediated sonodynamic therapy reverses macrophage and dendritic cell passivity in murine melanoma xenografts." in: Ultrasound in medicine & biology, Vol. 40, Issue 9, pp. 2125-33, (2014) (PubMed).

Ishida, Harada, Fukuda, Taguchi, Yagita, Fukushima: "Inhibition of very late antigen-4 and leukocyte function-associated antigen-1 in experimental autoimmune uveoretinitis." in: Clinical immunology (Orlando, Fla.), Vol. 153, Issue 1, pp. 136-44, (2014) (PubMed).

Huang, Li, Lin, Shi, Lin, Li, Xu: "Upregulation of thyroid transcription factor-1 and human leukocyte antigen class I in Hashimoto's disease providing a clinical evidence for possible triggering autoimmune reaction." in: European journal of endocrinology / European Federation of Endocrine Societies, Vol. 164, Issue 5, pp. 795-800, (2011) (PubMed).

Target Details for Interferon gamma

Target: Interferon gamma (IFNG)

Alternative Name: IFN gamma

Background:

Synonyms: Ifg, IFN-g, Interferon gamma, IFN-gamma, Ifng

Background: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

Gene ID: 15978

UniProt: P01580

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy