Ras-Related Protein Rab-7a (RAB7) antibody

Details for Product No. ABIN6699197
Antigen
Reactivity
Human
Host
Rabbit
Clonality
Polyclonal
Application
Immunohistochemistry (IHC), Western Blotting (WB)
Options
Immunogen

Immunogen: Rab-7 Antibody was produced from whole rabbit serum prepared by repeated immunizations at the internal amino acids of human Rab-7 protein.

Immunogen Type: Peptide

Isotype IgG
Cross-Reactivity Human, Mouse (Murine)
Purification Anti-Rab-7 Antibody was purified by peptide affinity chromatography. A BLAST analysis was used to suggest cross-reactivity with Rab-7 from rat and New World monkey based on 100% homology with the immunizing sequence. Cross-reactivity with Anti-Rab-7 from other sources has not been determined.
Antigen
Alternative Name Rab-7
Background

Synonyms: RAB7, Ras-related protein Rab-7a

Background: Anti-Rab7 antibody detects human Rab7. Rab7 is a 207 amino acid transport carrier, belonging to the Rab family of RAS-related GTP-binding proteins with 4 GTP/GDP-binding sites and terminates in sequences such as cys-X-cys, cys-cys or a closely related sequence. Rab7 located in specific intracellular compartments is known to be an important regulator of vesicular transport of proteins from early to late endosomes and lysosome biogenesis. Rab7 accelerates thyroid hormone production controlling aggregation and fusion of late lysosomes. It is ubiquitously expressed in most of the tissues and plays an essential role in maintenance of the perinuclear lysosome compartment. Anti-Rab7 antibody is ideal for investigators involved in caner, NFkappaB , and signaling research, kinase and phosphatase protein research.

Gene Name: RAB7A

Gene ID 7879
NCBI Accession NP_004628
UniProt P51149
Application Notes

Immunohistochemistry Dilution: 10 μg/mL (paraffin)

Application Note: Anti-Rab7 antibody is suitable for use in IHC (p) and WB. Expect a band approximately 23 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 0.5-1 μg/mL

Restrictions For Research Use only
Format Liquid
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage RT,4 °C,-20 °C
Storage Comment Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Supplier Images
Image no. 1 for anti-Ras-Related Protein Rab-7a (RAB7) antibody (ABIN6699197) Rab-7 Immunohistochemistry. Immunohistochemistry of Rabbit Anti-Rab-7 antibody. Tissu...
Image no. 2 for anti-Ras-Related Protein Rab-7a (RAB7) antibody (ABIN6699197) Rab-7 Western Blot. Western Blot of Rabbit Anti-Rab-7 antibody. Lane 1: HeLa lysate w...
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