ADA antibody (AA 135-363)

Catalog No. ABIN6719272

This Mouse Monoclonal antibody specifically detects ADA in WB, IHC and FACS. It exhibits reactivity toward Human.

Quick Overview for ADA antibody (AA 135-363) (ABIN6719272)

Target: ADA (Adenosine Deaminase (ADA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ADA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: 6D4

Product Details anti-ADA Antibody

Binding Specificity: AA 135-363

Purpose: Anti-ADA Antibody (monoclonal, 6D4)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ADA Antibody (monoclonal, 6D4) (ABIN6719272). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: E. coli-derived human ADA recombinant protein (Position: Q135-L363). Human ADA shares 82.5% and 82.9% amino acid (aa) sequence identity with mouse and rat ADA, respectively.

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells

Comment:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ADA

Target: ADA (Adenosine Deaminase (ADA))

Alternative Name: ADA

Background:

Synonyms: Adenosine deaminase

Tissue Specificity: Found in all tissues, occurs in large amounts in T-lymphocytes (PubMed:20959412). Expressed at the time of weaning in gastrointestinal tissues.

Background: Adenosine Deaminase (also known as adenosine aminohydrolase, or ADA) is an enzyme involved in purine metabolism. Primarily, ADA in humans is involved in the development and maintenance of the immune system. However, ADA association has also been observed with epithelial cell differentiation, neurotransmission, and gestation maintenance. It has also been proposed that ADA, in addition to adenosine breakdown, stimulates release of excitatory amino acids and is necessary to the coupling of A1 adenosine receptors and heterotrimeric G proteins. Adenosine deaminase deficiency leads to pulmonary fibrosis, suggesting that chronic exposure to high levels of adenosine can exacerbate inflammation responses rather than suppressing them. It has also been recognized that adenosine deaminase protein and activity is upregulated in mouse hearts that overexpress HIF-1 alpha, which in part explains the attenuated levels of adenosine in HIF-1 alpha expressing hearts during ischemic stress.

Molecular Weight: 45 kDa

Gene ID: 100

UniProt: P00813

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Ribonucleoside Biosynthetic Process