PLG antibody

Catalog No. ABIN6719276

The Rabbit Polyclonal anti-PLG antibody has been validated for WB and FACS. It is suitable to detect PLG in samples from Human, Mouse and Rat.

Quick Overview for PLG antibody (ABIN6719276)

Target: PLG (Plasminogen (PLG))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLG antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-PLG Antibody

Purpose: Anti-Plasminogen/PLG Antibody Picoband®

Sequence: EPLDDYVNTQ GASLFSVTKK QL

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Plasminogen/PLG Antibody Picoband® (ABIN6719276). Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Plasminogen, which shares 72.7% amino acid (aa) sequence identity with both mouse and rat Plasminogen.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Forsgren, M., Raden, B., Israelsson, M., Larsson, K., Heden, L.-O. Molecular cloning and characterization of a full-length cDNA clone for human plasminogen. FEBS Lett. 213: 254-260, 1987. 2. Miyata, T., Iwanaga, S., Sakata, Y., Aoki, N. Plasminogen Tochigi: inactive plasmin resulting from replacement of alanine-600 by threonine in the active site. Proc. Nat. Acad. Sci. 79: 6132-6136, 1982.

Comment:

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for PLG

Target: PLG (Plasminogen (PLG))

Alternative Name: PLG

Background:

Synonyms: Plasminogen, Plasmin heavy chain A, Activation peptide, Angiostatin, Plasmin heavy chain A, short form, Plasmin light chain B, PLG

Tissue Specificity: Present in plasma and many other extracellular fluids. It is synthesized in the liver.

Background: Plasminogen (PLG) is a circulating zymogen that is converted to the active enzyme plasmin by cleavage of the peptide bond between arg560 and val561, which is mediated by urokinase (PLAU) and tissue plasminogen activator (PLAT). The main function of plasmin is to dissolve fibrin (see, e.g., FGA) clots. Plasmin, like trypsin, belongs to the family of serine proteinases. Defects in this gene are likely a cause of thrombophilia and ligneous conjunctivitis.

Molecular Weight: 100 kDa

Gene ID: 5340

UniProt: P00747

Pathways: Complement System, Lipid Metabolism