LY9 antibody

Catalog No. ABIN6719290

This Rabbit Polyclonal antibody specifically detects LY9 in FACS, IHC and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for LY9 antibody (ABIN6719290)

Target: LY9 (CD229) (Lymphocyte Antigen 9 (CD229))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LY9 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-LY9 Antibody

Purpose: Anti-CD229/LY9 Antibody

Sequence: YKAQINQRNF EVTTEEEFTL FVYEQLQEPQ VTMK

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-/LY9 Antibody Picoband® (ABIN6719290). Tested in Flow Cytometry, IHC, ICC applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human CD229/LY9, which shares 55.9% and 61.8% amino acid (aa) sequence identity with mouse and rat CD229/LY9, respectively.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
Immunocytochemistry, 0.5-1 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells

1. Sandrin MS, Henning MM, Lo MF, Baker E, Sutherland GR, McKenzie IF (Feb 1996). "Isolation and characterization of cDNA clones for Humly9: the human homologue of mouse Ly9". Immunogenetics. 2. Kingsmore SF, Souryal CA, Watson ML, Patel DD, Seldin MF (Aug 1995). "Physical and genetic linkage of the genes encoding Ly-9 and CD48 on mouse and human chromosomes 1". Immunogenetics. 3. "Entrez Gene: LY9 lymphocyte antigen 9".

Comment:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for LY9

Target: LY9 (CD229) (Lymphocyte Antigen 9 (CD229))

Alternative Name: LY9

Background:

Synonyms: T-lymphocyte surface antigen Ly-9, Cell surface molecule Ly-9, Lymphocyte antigen 9, SLAM family member 3, SLAMF3, Signaling lymphocytic activation molecule 3, CD229, LY9, CDABP0070

Tissue Specificity: Increased surface expression on T-cells of systemic lupus erythematosus (SLE) patients.

Background: T-lymphocyte surface antigen Ly-9 is a protein that in humans is encoded by the LY9 gene. This gene is mapped to 17q21.31. LY9 has also recently been designated CD229 (cluster of differentiation 229). LY9 belongs to the SLAM family of immunomodulatory receptors and interacts with the adaptor molecule SAP.

Molecular Weight: 28 kDa

Gene ID: 4063

Pathways: BCR Signaling