CSTB antibody (AA 1-98) (DyLight 550)

Catalog No. ABIN6719308

This Mouse Monoclonal antibody specifically detects CSTB in FACS. It exhibits reactivity toward Human.

Quick Overview for CSTB antibody (AA 1-98) (DyLight 550) (ABIN6719308)

Target: CSTB (Cystatin B (Stefin B) (CSTB))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CSTB antibody is conjugated to DyLight 550

Application: Flow Cytometry (FACS)

Clone: 2B6

Product Details anti-CSTB Antibody (DyLight 550)

Binding Specificity: AA 1-98

Purpose: Anti-Human Stefin B DyLight® 550 conjugated CSTB Antibody(monoclonal, 2B6)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Human Stefin B DyLight® 550 conjugated CSTB Antibody (monoclonal, 2B6) (ABIN5692840)-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human Stefin B recombinant protein (Position: M1-F98). Human Stefin B shares 78.6 % amino acid (aa) sequence identity with both mouse and rat Stefin B.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells1. Alakurtti, K., Weber, E., Rinne, R., Theil, G., de Haan, G.-J., Lindhout, D., Salmikangas, P., Saukko, P., Lahtinen, U., Lehesjoki, A.-E. Loss of lysosomal association of cystatin B proteins representing progressive myoclonus epilepsy, EPM1, mutations. Europ. J. Hum. Genet. 13: 208-215, 2005. Note: Erratum: Europ. J. Hum. Genet. 13: 264 only, 2005. 2. Antonarakis, S. Personal Communication. Geneva, Switzerland 4/8/1997. 3. Bespalova, I. N., Adkins, S., Pranzatelli, M., Burmeister, M. Novel cystatin B mutation and diagnostic PCR assay in an Unverricht-Lundborg progressive myoclonus epilepsy patient. Am. J. Med. Genet. 74: 467-471, 1997.

Comment:

Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Target Details for CSTB

Target: CSTB (Cystatin B (Stefin B) (CSTB))

Alternative Name: CSTB

Background:

Synonyms: Cystatin-B, CPI-B, Liver thiol proteinase inhibitor, Stefin-B, CSTB, CST6, STFB

Background: Cystatin B (CSTB), also called STFB, is a small protein that is a member of the superfamily of cysteine protease inhibitors. It has been isolated from human spleen and liver and its amino acid sequence has been fully determined. The cystatin B gene is located on 21q22.3. It is widely distributed and is localized mostly intracellularly, but has been found extracellularly. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. Its role is thought to be as a protector against the proteinases leaking from lysosomes. A cystatin B multiprotein complex might have a specific cerebellar function, and that the loss of this function might contribute to the etiopathogenesis of EPM1. Upon differentiation to myotubes, CSTB becomes excluded from the nucleus and lysosomes, suggesting that the subcellular distribution of CSTB is dependent on the differentiation status of the cell.

Molecular Weight: 39 kDa

Gene ID: 1476

UniProt: P04080

Pathways: Response to Water Deprivation