Dynamin 1 antibody (AA 616-667) (DyLight 550)

Catalog No. ABIN7354731

This Rabbit Polyclonal antibody specifically detects Dynamin 1 in FACS. It exhibits reactivity toward Human.

Quick Overview for Dynamin 1 antibody (AA 616-667) (DyLight 550) (ABIN7354731)

Target: Dynamin 1 (DNM1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Dynamin 1 antibody is conjugated to DyLight 550

Application: Flow Cytometry (FACS)

Product Details anti-Dynamin 1 Antibody (DyLight 550)

Binding Specificity: AA 616-667

Purpose: Anti-Human Dynamin 1 DyLight® 550 conjugated DNM1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Human Dynamin 1 DyLight® 550 conjugated DNM1 Antibody -Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human Dynamin 1 recombinant protein (Position: W616-D667).

Isotype: IgG

Application Details

Application Notes: Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells1. Dhindsa, R. S., Bradrick, S. S., Yao, X., Heinzen, E. L., Petrovski, S., Krueger, B. J., Johnson, M. R., Frankel, W. N., Petrou, S., Boumil, R. M., Goldstein, D. B. Epileptic encephalopathy-causing mutations in DNM1 impair synaptic vesicle endocytosis. Neurol. Genet. 1: e4, 2015. 2. EuroEPINOMICS-RES Consortium, Epilepsy Phenome/Genome Project, Epi4K Consortium. De novo mutations in synaptic transmission genes including DNM1 cause epileptic encephalopathies. Am. J. Hum. Genet. 95: 360-370, 2014.

Comment:

Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Target Details for Dynamin 1

Target: Dynamin 1 (DNM1)

Alternative Name: DNM1

Background:

Synonyms: Dynamin-1, DNM1, DNM

Background: Dynamin-1 is a protein that in humans is encoded by the DNM1 gene. This gene encodes a member of the dynamin subfamily of GTP-binding proteins. The encoded protein possesses unique mechanochemical properties used to tubulate and sever membranes, and is involved in clathrin-mediated endocytosis and other vesicular trafficking processes. Actin and other cytoskeletal proteins act as binding partners for the encoded protein, which can also self-assemble leading to stimulation of GTPase activity. More than sixty highly conserved copies of the 3' region of this gene are found elsewhere in the genome, particularly on chromosomes Y and 15. Alternatively spliced transcript variants encoding different isoforms have been described.

Molecular Weight: 39 kDa

Gene ID: 1759

UniProt: Q05193

Pathways: Toll-Like Receptors Cascades, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling