Emerin antibody (N-Term) (DyLight 550)

Catalog No. ABIN6719312

The Mouse Monoclonal anti-Emerin antibody has been validated for FACS. It is suitable to detect Emerin in samples from Human.

Quick Overview for Emerin antibody (N-Term) (DyLight 550) (ABIN6719312)

Target: Emerin (EMD)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Emerin antibody is conjugated to DyLight 550

Application: Flow Cytometry (FACS)

Clone: 5A10

Product Details anti-Emerin Antibody (DyLight 550)

Binding Specificity: AA 1-48, N-Term

Purpose: Anti-Human Emerin DyLight® 550 conjugated EMD Antibody(monoclonal, 5A10)

Sequence: MDNYADLSDT ELTTLLRRYN IPHGPVVGST RRLYEKKIFE YETQRRRL

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Human Emerin DyLight® 550 conjugated EMD Antibody (monoclonal, 5A10) (ABIN5692852)-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Emerin, different from the related mouse sequence by eight amino acids, and from the related rat sequence by nine amino acids.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells1. "Entrez Gene: EMD emerin (Emery-Dreifuss muscular dystrophy)". 2. Lammerding, J, Hsiao, J, Schulze, PC, Kozlov, S, Stewart, CL, Lee, RT (29 August 2005). "Abnormal nuclear shape and impaired mechanotransduction in emerin-deficient cells.". The Journal of Cell Biology. 170 (5): 781-91. 3. Nagano A, Koga R, Ogawa M, Kurano Y, Kawada J, Okada R, Hayashi YK, Tsukahara T, Arahata K (Mar 1996). "Emerin deficiency at the nuclear membrane in patients with Emery-Dreifuss muscular dystrophy". Nature Genetics. 12 (3): 254-9.

Comment:

Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Target Details for Emerin

Target: Emerin (EMD)

Alternative Name: EMD

Background:

Synonyms: Emerin, EMD, EDMD, STA

Tissue Specificity: Skeletal muscle, heart, colon, testis, ovary and pancreas.

Molecular Weight: 39 kDa

Gene ID: 2010

UniProt: P50402