IL-22 antibody (AA 34-179) (DyLight 550)

Catalog No. ABIN6719332

This Mouse Monoclonal antibody specifically detects IL-22 in FACS. It exhibits reactivity toward Human.

Quick Overview for IL-22 antibody (AA 34-179) (DyLight 550) (ABIN6719332)

Target: IL-22 (IL22) (Interleukin 22 (IL22))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-22 antibody is conjugated to DyLight 550

Application: Flow Cytometry (FACS)

Clone: 7F2

Product Details anti-IL-22 Antibody (DyLight 550)

Binding Specificity: AA 34-179

Purpose: Anti-Human IL22 DyLight® 550 conjugated Antibody(monoclonal, 7F2)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Human IL22 DyLight® 550 conjugated Antibody (monoclonal, 7F2) (ABIN6719333)-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human IL-22 recombinant protein (Position: A34-I179). Human IL-22 shares 81.4% amino acid (aa) sequence identity with mouse IL-22.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells1. Dumoutier, L., Van Roost, E., Ameye, G., Michaux, L., Renauld, J.-C. IL-TIF/IL-22: genomic organization and mapping of the human and mouse genes. Genes Immunity 1: 488-494, 2000. 2. Moore KW, de Waal Malefyt R, Coffman RL, O'Garra A (2001). "Interleukin-10 and the interleukin-10 receptor". Annu. Rev. Immunol. 19: 683-765.

Comment:

Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: Lot specific

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Target Details for IL-22

Target: IL-22 (IL22) (Interleukin 22 (IL22))

Alternative Name: IL22

Background:

Synonyms: Interleukin-22, IL-22, Cytokine Zcyto18, IL-10-related T-cell-derived-inducible factor, IL-TIF, IL22, ILTIF, ZCYTO18, UNQ3099/PRO10096

Tissue Specificity: Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland.

Background: Interleukin-22 (IL-22), also known as ILTIF, is protein that in humans is encoded by the IL22 gene. IL-22 a member of a group of cytokines called the IL-10 family or IL-10 superfamily, a class of potent mediators of cellular inflammatory responses. Using FISH, the IL22 gene is mapped to chromosome 12q15, close to the IFNG and the herpesvirus saimiri-induced AK155 genes. IL-22 can contribute to immune disease through the stimulation of inflammatory responses, S100s and defensins. It also promotes hepatocyte survival in the liver and epithelial cells in the lung and gut similar to IL-10. In some contexts, the pro-inflammatory versus tissue-protective functions of IL-22 are regulated by the often co-expressed cytokine IL-17A.

Molecular Weight: 39 kDa

Gene ID: 50616