LUM antibody (AA 50-338)

Catalog No. ABIN6719339

The Rabbit Polyclonal anti-LUM antibody has been validated for WB, IHC and ELISA. It is suitable to detect LUM in samples from Human, Rat and Mouse.

Quick Overview for LUM antibody (AA 50-338) (ABIN6719339)

Target: LUM (Lumican (LUM))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LUM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-LUM Antibody

Binding Specificity: AA 50-338

Purpose: Anti-Lumican Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Lumican Antibody Picoband® (ABIN6719339). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human Lumican recombinant protein (Position: A50-N338).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
ELISA, 0.1-0.5 μg/mL
1. "Entrez Gene: LUM lumican". 2. Chakravarti S, Stallings RL, SundarRaj N, Cornuet PK, Hassell JR (Jun 1995). "Primary structure of human lumican (keratan sulfate proteoglycan) and localization of the gene (LUM) to chromosome 12q21.3-q22". Genomics27 (3): 481-8. 3. Chakravarti S (2002). "Functions of lumican and fibromodulin: lessons from knockout mice". Glycoconjugate Journal 19 (4-5): 287-93.

Comment:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for LUM

Target: LUM (Lumican (LUM))

Alternative Name: LUM

Background:

Synonyms: Lumican, Keratan sulfate proteoglycan lumican, KSPG lumican, LUM, LDC, SLRR2D,

Tissue Specificity: Cornea and other tissues.

Background: Lumican, also known as LUM, is an extracellular matrix protein that, in humans, is encoded by the LUM gene on chromosome 12. Lumican is a major keratan sulfate proteoglycan of the cornea but is ubiquitously distributed in most mesenchymal tissues throughout the body. And Lumican is involved in collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair. Corneal transparency is possible due to the exact alignment of collagen fibers by lumican (and keratocan) in the intrafibrillar space.

Molecular Weight: 58 kDa

Gene ID: 4060

UniProt: P51884

Pathways: Glycosaminoglycan Metabolic Process