RASAL1 antibody (AA 721-798)

Catalog No. ABIN6719367

This anti-RASAL1 antibody is a Rabbit Polyclonal antibody detecting RASAL1 in WB, ELISA and IHC. Suitable for Human, Mouse and Rat.

Quick Overview for RASAL1 antibody (AA 721-798) (ABIN6719367)

Target: RASAL1 (RAS Protein Activator Like 1 (GAP1 Like) (RASAL1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RASAL1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-RASAL1 Antibody

Binding Specificity: AA 721-798

Purpose: Anti-RASAL1/RASAL1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RASAL1/RASAL1 Antibody Picoband® (ABIN6719367). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human RASAL1 recombinant protein (Position: D721-A798).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Allen, M., Chu, S., Brill, S., Stotler, C., Buckler, A. Restricted tissue expression pattern of a novel human rasGAP-related gene and its murine ortholog. Gene 218: 17-25, 1998. 2. Bechtel, W., McGoohan, S., Zeisberg, E. M., Muller, G. A., Kalbacher, H., Salant, D. J., Muller, C. A., Kalluri, R., Zeisberg, M. Methylation determines fibroblast activation and fibrogenesis in the kidney. Nature Med. 16: 544-550, 2010. 3. Jin, H., Wang, X., Ying, J., Wong, A. H. Y., Cui, Y., Srivastava, G., Shen, Z.-Y., Li, E.-M., Zhang, Q., Jin, J., Kupzig, S., Chan, A. T. C., Cullen, P. J., Tao, Q. Epigenetic silencing of a Ca(2+)-regulated Ras GTPase-activating protein RASAL defines a new mechanism of Ras activation in human cancers. Proc. Nat. Acad. Sci. 104: 12353-12358, 2007.

Comment:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for RASAL1

Target: RASAL1 (RAS Protein Activator Like 1 (GAP1 Like) (RASAL1))

Alternative Name: RASAL1

Background:

Synonyms: RasGAP-activating-like protein 1, RAS protein activator like 1

Tissue Specificity: Highly expressed in thyroid and adrenal medulla, lower expression in brain, spinal cord and trachea (PubMed:9751798). Expressed in melanocytes (PubMed:23999003).

Background: The protein encoded by this gene is member of the GAP1 family of GTPase-activating proteins. And it is mapped to 12q24.13. These proteins stimulate the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. This particular family member contains domains which are characteristic of the GAP1 subfamily of RasGAP proteins but, in contrast to the other GAP1 family members, this protein is strongly and selectively expressed in endocrine tissues. Alternatively spliced transcript variants that encode different isoforms have been described.

Molecular Weight: 90 kDa

Gene ID: 8437

UniProt: O95294