SYT1 antibody (DyLight 550)

Catalog No. ABIN7354719

This Rabbit Polyclonal antibody specifically detects SYT1 in FACS. It exhibits reactivity toward Human.

Quick Overview for SYT1 antibody (DyLight 550) (ABIN7354719)

Target: SYT1 (Synaptotagmin I (SYT1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SYT1 antibody is conjugated to DyLight 550

Application: Flow Cytometry (FACS)

Product Details anti-SYT1 Antibody (DyLight 550)

Purpose: Anti-Human Synaptotagmin 1 DyLight® 550 conjugated SYT1 Antibody

Sequence: RRPIAQWHTL QVEEEVDAML AVKK

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Human Synaptotagmin 1 DyLight® 550 conjugated SYT1 Antibody -Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Synaptotagmin 1, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells1. Fernandez-Chacon, R., Konigstorfer, A., Gerber, S. H., Garcia, J., Matos, M. F., Stevens, C. F., Brose, N., Rizo, J., Rosenmund, C., Sudhof, T. C. Synaptotagmin I functions as a calcium regulator of release probability. Nature 410: 41-49, 2001. 2. Poskanzer, K. E., Marek, K. W., Sweeney, S. T., Davis, G. W. Synaptotagmin I is necessary for compensatory synaptic vesicle endocytosis in vivo. Nature 426: 559-563, 2003.

Comment:

Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Target Details for SYT1

Target: SYT1 (Synaptotagmin I (SYT1))

Alternative Name: SYT1

Background:

Synonyms: Synaptotagmin-1, Synaptotagmin I, SytI, p65, SYT1, SVP65, SYT

Tissue Specificity: Expressed in melanocytes (PubMed:23999003).

Background: Synaptotagmin-1 is a protein that in humans is encoded by the SYT1 gene. The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca (2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse.

Molecular Weight: 39 kDa

Gene ID: 6857

UniProt: P21579

Pathways: Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport