ITGA6 antibody (AA 189-917)

Catalog No. ABIN6719411

The Rabbit Polyclonal anti-ITGA6 antibody has been validated for WB, FACS and ELISA. It is suitable to detect ITGA6 in samples from Human, Mouse and Rat.

Quick Overview for ITGA6 antibody (AA 189-917) (ABIN6719411)

Target: ITGA6 (Integrin, alpha 6 (ITGA6))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ITGA6 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA

Product Details anti-ITGA6 Antibody

Binding Specificity: AA 189-917

Purpose: Anti-Integrin alpha 6/ITGA6 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Integrin alpha 6/ITGA6 Antibody Picoband® (ABIN6719411). Tested in Flow Cytometry, ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Integrin alpha 6/ITGA6 recombinant protein (Position: Q189-K917).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5 μg/mL
1. Notta, F., Doulatov, S., Laurenti, E., Poeppl, A., Jurisica, I., Dick, J. E. Isolation of single human hematopoietic stem cells capable of long-term multilineage engraftment. Science 333: 218-221, 2011.  2. Ruzzi, L., Gagnoux-Palacios, L., Pinola, M., Belli, S., Meneguzzi, G., Zambruno, G., D'Alessio, M. A homozygous mutation in the gene encoding integrin alpha6 in junctional epidermolysis bullosa with pyloric atresia. (Abstract) Medizinische Genetik 9: 13-14, 1997.

Comment:

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ITGA6

Target: ITGA6 (Integrin, alpha 6 (ITGA6))

Alternative Name: ITGA6

Background:

Synonyms: Integrin alpha-6, CD49 antigen-like family member F, VLA-6, CD49f, Alpha6p, ITGA6

Tissue Specificity: Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines, they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney).

Background: Integrin alpha-6, also called VLA-6 or ITGA6B is a protein that in humans is encoded by the ITGA6 gene. This gene is mapped to 2q31.1. The ITGA6 protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome.

Molecular Weight: 127 kDa

Gene ID: 3655

UniProt: P23229

Pathways: CXCR4-mediated Signaling Events, Brown Fat Cell Differentiation, Integrin Complex