STUB1 antibody (AA 51-303)

Catalog No. ABIN6719414

The Mouse Monoclonal anti-STUB1 antibody has been validated for WB, IHC and FACS. It is suitable to detect STUB1 in samples from Human, Mouse, Rat and Monkey.

Quick Overview for STUB1 antibody (AA 51-303) (ABIN6719414)

Target: STUB1 (STIP1 Homology and U-Box Containing Protein 1 (STUB1))

Reactivity: Human, Mouse, Rat, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This STUB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: 13I8

Product Details anti-STUB1 Antibody

Binding Specificity: AA 51-303

Purpose: Anti-STUB1 Antibody Picoband® (monoclonal, 13I8)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-STUB1 Antibody Picoband® (monoclonal, 13I8) (ABIN6719414). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human STUB1 recombinant protein (Position: R51-Y303).

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
1."Entrez Gene: STUB1 STIP1 homology and U-box containing protein 1". 2.Heimdal, K., Sanchez-Guixe, M., Aukrust, I., Bollerslev, J., Bruland, O., Jablonski, G. E., Erichsen, A. K., Gude, E., Koht, J. A., Erdal, S., Fiskerstrand, T., Haukanes, B. I., Boman, H., Bjorkhaug, L., Tallaksen, C. M. E., Knappskog, P. M., Johansson, S. STUB1 mutations in autosomal recessive ataxias--evidence for mutation-specific clinical heterogeneity. Orphanet J. Rare Dis. 9: 146, 2014.

Comment:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for STUB1

Target: STUB1 (STIP1 Homology and U-Box Containing Protein 1 (STUB1))

Alternative Name: STUB1

Background:

Synonyms: E3 ubiquitin-protein ligase CHIP, Antigen NY-CO-7, CLL-associated antigen KW-8, Carboxy terminus of Hsp70-interacting protein, RING-type E3 ubiquitin transferase CHIP, STIP1 homology and U box-containing protein 1, STUB1, CHIP, PP1131

Tissue Specificity: Expressed in differentiated myotubes (at protein level). Highly expressed in skeletal muscle, heart, pancreas, brain and placenta . Detected in kidney, liver and lung.

Background: STUB1 (STIP1 homology and U-Box containing protein 1), also known as CHIP (C terminus of HSC70-Interacting Protein), is a human gene. This gene encodes a protein containing tetratricopeptide repeat and a U-box that functions as a ubiquitin ligase/cochaperone. The encoded protein binds to and ubiquitinates shock cognate 71 kDa protein (Hspa8) and DNA polymerase beta (Polb), among other targets. Mutations in this gene cause spinocerebellar ataxia, autosomal recessive 16. Alternative splicing results in multiple transcript variants. There is a pseudogene for this gene on chromosome 2.

Molecular Weight: 35 kDa

Gene ID: 10273

Pathways: Regulation of Hormone Metabolic Process, Response to Water Deprivation