LCP1 antibody

Catalog No. ABIN6719421

This anti-LCP1 antibody is a Rabbit Polyclonal antibody detecting LCP1 in WB, ELISA, IHC, IF, ICC and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for LCP1 antibody (ABIN6719421)

Target: LCP1 (Lymphocyte Cytosolic Protein 1 (LCP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LCP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-LCP1 Antibody

Purpose: Anti-Plastin L/LCP1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Plastin L/LCP1 Antibody Picoband® (ABIN6719421). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human Plastin L/LCP1 recombinant protein (M1-K444).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5 μg/mL
1. Chen, H., Mocsai, A., Zhang, H., Ding, R.-X., Morisaki, J. H., White, M., Rothfork, J. M., Heiser, P., Colucci-Guyon, E., Lowell, C. A., Gresham, H. D., Allen, P. M., Brown, E. J. Role for plastin in host defense distinguishes integrin signaling from cell adhesion and spreading. Immunity 19: 95-104, 2003. 2. Galiegue-Zouitina, S., Quief, S., Hildebrand, M.-P., Denis, C., Detourmignies, L., Lai, J.-L., Kerckaert, J.-P. Nonrandom fusion of L-plastin (LCP1) and LAZ3 (BCL6) genes by t(3,13)(q27,q14) chromosome translocation in two cases of B-cell non-Hodgkin lymphoma. Genes Chromosomes Cancer 26: 97-105, 1999. 3. Giometti, C. S., Anderson, N. L. A variant of human nonmuscle tropomyosin found in fibroblasts by using two-dimensional electrophoresis. J. Biol. Chem. 256: 11840-11846, 1981.

Comment:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for LCP1

Target: LCP1 (Lymphocyte Cytosolic Protein 1 (LCP1))

Alternative Name: LCP1

Background:

Synonyms: Plastin-2, L-plastin, LC64P, Lymphocyte cytosolic protein 1, LCP-1, LCP1, PLS2

Tissue Specificity: Detected in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia, in spleen and other lymph node-containing organs. Expressed in peripheral blood T-lymphocytes, neutrophils, monocytes, B-lymphocytes, and myeloid cells.

Background: Plastin-2 is a protein that in humans is encoded by the LCP1 gene. This gene is mapped to 13q14.13. Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). However, L-plastin has been found in many types of malignant human cells of non-hemopoietic origin suggesting that its expression is induced accompanying tumorigenesis in solid tissues.

Molecular Weight: 70 kDa

Gene ID: 3936

UniProt: P13796