KIF20B antibody (AA 19-231)

Catalog No. ABIN6719429

The Rabbit Polyclonal anti-KIF20B antibody has been validated for ELISA and WB. It is suitable to detect KIF20B in samples from Human, Mouse and Rat.

Quick Overview for KIF20B antibody (AA 19-231) (ABIN6719429)

Target: KIF20B (Kinesin Family Member 20B (KIF20B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KIF20B antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-KIF20B Antibody

Binding Specificity: AA 19-231

Purpose: Anti-MPP1/KIF20B Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MPP1/KIF20B Antibody Picoband® (ABIN6719429). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MPP1/KIF20B recombinant protein (Position: A19-D231).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Kamimoto, T., Zama, T., Aoki, R., Muro, Y., Hagiwara, M. Identification of a novel kinesin-related protein, KRMP1, as a target for mitotic peptidyl-prolyl isomerase Pin1. J. Biol. Chem. 276: 37520-37528, 2001. 2. Matsumoto-Taniura, N., Pirollet, F., Monroe, R., Gerace, L., Westendorf, J. M. Identification of novel M phase phosphoproteins by expression cloning. Molec. Biol. Cell 7: 1455-1469, 1996. 3. Westendorf, J. M., Rao, P. N., Gerace, L. Cloning of cDNAs for M-phase phosphoproteins recognized by the MPM2 monoclonal antibody and determination of the phosphorylated epitope. Proc. Nat. Acad. Sci. 91: 714-718, 1994.

Comment:

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for KIF20B

Target: KIF20B (Kinesin Family Member 20B (KIF20B))

Alternative Name: KIF20B

Background:

Synonyms: Kinesin-like protein KIF20B, Cancer/testis antigen 90, CT90, Kinesin family member 20B, Kinesin-related motor interacting with PIN1, M-phase phosphoprotein 1, MPP1, KIF20B, KRMP1

Tissue Specificity: Brain, ovary, kidney and testis. Overexpressed in bladder cancer cells. Expressed in testis. Overexpressed in bladder cancer cells.

Background: Kinesin-like protein KIF20B is a protein that in humans is encoded by the KIF20B gene. The MPP1 gene is previously labeled as KIF20B. Palmitoylated membrane protein 1 is the prototype of a family of membrane-associated proteins termed MAGUKs (membrane-associated guanylate kinase homologs). MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. Palmitoylated membrane protein 1 contains a conserved sequence, called the SH3 (src homology 3) motif, found in several other proteins that associate with the cytoskeleton and are suspected to play important roles in signal transduction.

Molecular Weight: 211 kDa

Gene ID: 9585

UniProt: Q96Q89

Pathways: M Phase