NMI antibody (AA 2-307)

Catalog No. ABIN6719489

The Mouse Monoclonal anti-NMI antibody has been validated for WB, IHC, IF, FACS and ICC. It is suitable to detect NMI in samples from Human, Rat and Mouse.

Quick Overview for NMI antibody (AA 2-307) (ABIN6719489)

Target: NMI (N-Myc (And STAT) Interactor (NMI))

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This NMI antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 2F3

Product Details anti-NMI Antibody

Binding Specificity: AA 2-307

Purpose: Anti-NMI Antibody Picoband® (monoclonal, 2F3)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NMI Antibody Picoband® (monoclonal, 2F3) (ABIN6719489). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NMI recombinant protein (Position: E2-E307). Human NMI shares 64% amino acid (aa) sequence identity with mouse NMI.

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 5 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
1.Albihn A, et al. Camptothecin-induced apoptosis is enhanced by Myc and involves PKCdelta signaling. Int J Cancer, 2007 Oct 15. 2.Fillmore RA, et al. Nmi (N-Myc interactor) inhibits Wnt/beta-catenin signaling and retards tumor growth. Int J Cancer, 2009 Aug 1. 3.Lebrun SJ, et al. Interferon-induced upregulation and cytoplasmic localization of Myc-interacting protein Nmi. J Interferon Cytokine Res, 1998 Sep.

Comment:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for NMI

Target: NMI (N-Myc (And STAT) Interactor (NMI))

Alternative Name: NMI

Background:

Synonyms: N-myc-interactor, Nmi, N-myc and STAT interactor, NMI

Tissue Specificity: Expressed in all adult and fetal tissues except brain and skin. More abundant in fetal tissues especially liver.

Background: NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has low expression levels in all human fetal and adult tissues tested except brain and has high expression in cancer cell line-myeloid leukemias.

Molecular Weight: 40 kDa

Gene ID: 9111

UniProt: Q13287