CD2AP antibody (AA 253-337) (DyLight 550)

Catalog No. ABIN7354681

This Rabbit Polyclonal antibody specifically detects CD2AP in FACS. It exhibits reactivity toward Human.

Quick Overview for CD2AP antibody (AA 253-337) (DyLight 550) (ABIN7354681)

Target: CD2AP (Cd2ap) (CD2-Associated Protein (Cd2ap))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD2AP antibody is conjugated to DyLight 550

Application: Flow Cytometry (FACS)

Product Details anti-CD2AP Antibody (DyLight 550)

Binding Specificity: AA 253-337

Purpose: Anti-Human CD2AP DyLight® 550 conjugated Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Human CD2AP DyLight® 550 conjugated Antibody -Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human CD2AP recombinant protein (Position: K253-K337).

Isotype: IgG

Application Details

Application Notes: Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells1. Cochran, J. N., Rush, T., Buckingham, S. C., Roberson, E. D. The Alzheimer's disease risk factor CD2AP maintains blood-brain barrier integrity. Hum. Molec. Genet. 24: 6667-6674, 2015. 2. Lowik, M. M., Groenen, P. J. T. A., Pronk, I., Lilien, M. R., Goldschmeding, R., Dijkman, H. B., Levtchenko, E. N., Monnens, L. A., van den Heuvel, L. P. Focal segmental glomerulosclerosis in a patient homozygous for a CD2AP mutation. Kidney Int. 72: 1198-1203, 2007.

Comment:

Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Target Details for CD2AP

Target: CD2AP (Cd2ap) (CD2-Associated Protein (Cd2ap))

Alternative Name: CD2AP

Background:

Synonyms: CD2-associated protein, Adapter protein CMS, Cas ligand with multiple SH3 domains, CD2AP

Tissue Specificity: Widely expressed in fetal and adult tissues.

Background: CD2-associated protein is a protein that in humans is encoded by the CD2AP gene. This gene encodes a scaffolding molecule that regulates the actin cytoskeleton. The protein ly interacts with filamentous actin and a variety of cell membrane proteins through multiple actin binding sites, SH3 domains, and a proline-rich region containing binding sites for SH3 domains. The cytoplasmic protein localizes to membrane ruffles, lipid rafts, and the leading edges of cells. It is implicated in dynamic actin remodeling and membrane trafficking that occurs during receptor endocytosis and cytokinesis. Haploinsufficiency of this gene is implicated in susceptibility to glomerular disease.

Molecular Weight: 39 kDa

Gene ID: 23607

UniProt: Q9Y5K6