XPO1 antibody (AA 966-1071) (DyLight 550)

Catalog No. ABIN6719560

This Mouse Monoclonal antibody specifically detects XPO1 in FACS. It exhibits reactivity toward Human.

Quick Overview for XPO1 antibody (AA 966-1071) (DyLight 550) (ABIN6719560)

Target: XPO1 (Exportin 1 (XPO1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This XPO1 antibody is conjugated to DyLight 550

Application: Flow Cytometry (FACS)

Clone: 5G3

Product Details anti-XPO1 Antibody (DyLight 550)

Binding Specificity: AA 966-1071

Purpose: Anti-Human CRM1 DyLight® 550 conjugated XPO1 Antibody(monoclonal, 5G3)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Human CRM1 DyLight® 550 conjugated XPO1 Antibody (monoclonal, 5G3) (ABIN5693213)-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CRM1 recombinant protein (Position: N966-D1071). Human CRM1 shares 93.4% and 91.5% amino acid (aa) sequence identity with mouse and rat CRM1, respectively.

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells1. Dong, X., Biswas, A., Suel, K. E., Jackson, L. K., Martinez, R., Gu, H., Chook, Y. M. Structural basis for leucine-rich nuclear export signal recognition by CRM1. Nature 458: 1136-1141, 2009. Note: Erratum: Nature 461: 550 only, 2009. 2. Fornerod, M., van Baal, S., Valentine, V., Shapiro, D. N., Grosveld, G. Chromosomal localization of genes encoding CAN/Nup214-interacting proteins--human CRM1 localizes to 2p16, whereas Nup88 localizes to 17p13 and is physically linked to SF2p32. Genomics 42: 538-540, 1997. 3. Kudo, N., Khochbin, S., Nishi, K., Kitano, K., Yanagida, M., Yoshida, M., Horinouchi, S. Molecular cloning and cell cycle-dependent expression of mammalian CRM1, a protein involved in nuclear export of proteins. J. Biol. Chem. 272: 29742-29751, 1997.

Comment:

Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: Lot specific

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Target Details for XPO1

Target: XPO1 (Exportin 1 (XPO1))

Alternative Name: XPO1

Background:

Synonyms: Exportin-1, Exp1, Chromosome region maintenance 1 protein homolog, XPO1, CRM1

Tissue Specificity: Expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. Not expressed in the kidney.

Background: Exportin 1 (XPO1), also known as chromosomal maintenance 1 (CRM1), is an eukaryotic protein that mapped to human chromosome 2p16 by fluorescence in situ hybridization. This protein mediates leucine-rich nuclear export signal (NES)-dependent protein transport. It specifically inhibits the nuclear export of Rev and U snRNAs. Additionally, this protein is involved in the control of several cellular processes by controlling the localization of cyclin B, MPAK, and MAPKAP kinase 2. It also regulates NFAT and AP-1.

Molecular Weight: 39 kDa

Gene ID: 7514

UniProt: O14980

Pathways: M Phase