DIS3 antibody (AA 62-304)

Catalog No. ABIN6719579

The Rabbit Polyclonal anti-DIS3 antibody has been validated for WB, ELISA, IP, IF, ICC and FACS. It is suitable to detect DIS3 in samples from Human and Rat.

Quick Overview for DIS3 antibody (AA 62-304) (ABIN6719579)

Target: DIS3 (Exosome Complex Exonuclease RRP44 (DIS3))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DIS3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-DIS3 Antibody

Binding Specificity: AA 62-304

Purpose: Anti-DIS3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-DIS3 Antibody (ABIN6719579). Tested in ELISA, Flow Cytometry, IP, IF, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human DIS3 recombinant protein (Position: Q62-A304).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunocytochemistry/Immunofluorescence, 5 μg/mL
Immunoprecipitation, 0.5-2 μg/mL, Human
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5 μg/mL
1. Nagase, T., Ishikawa, K., Suyama, M., Kikuno, R., Hirosawa, M., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., Ohara, O. Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 6: 63-70, 1999. 2. Rozenblum, E., Vahteristo, P., Sandberg, T., Bergthorsson, J. T., Syrjakoski, K., Weaver, D., Haraldsson, K., Johannsdottir, H. K., Vehmanen, P., Nigam, S., Golberger, N., Robbins, C., and 12 others. A genomic map of a 6-Mb region at 13q21-q22 implicated in cancer development: identification and characterization of candidate genes. Hum. Genet. 110: 111-121, 2002. 3. Tomecki, R., Kristiansen, M. S., Lykke-Andersen, S., Chlebowski, A., Larsen, K. M., Szczesny, R. J., Drazkowska, K., Pastula, A., Andersen, J. S., Stepien, P. P., Dziembowski, A., Jensen, T. H. The human core exosome interacts with differentially localized processive RNases: hDIS3 and hDIS3L. EMBO J. 29: 2342-2357, 2010.

Comment:

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for DIS3

Target: DIS3 (Exosome Complex Exonuclease RRP44 (DIS3))

Alternative Name: DIS3

Background:

Synonyms: Exosome complex exonuclease RRP44, Protein DIS3 homolog, Ribosomal RNA-processing protein 44, DIS3, KIAA1008, RRP44

Tissue Specificity: Widely expressed.

Background: Exosome complex exonuclease RRP44 or Dis3 is an enzyme that in humans is encoded by the DIS3 gene. Its protein product is an RNase enzyme homologous to the yeast protein Rrp44, and can be part of the exosome complex in the nucleus of eukaryotic cells. By genomic sequence analysis, this gene is mapped to chromosome 13q21-q22. Expression of human DIS3 could partially complement Dis3 depletion in yeast.

Molecular Weight: 109 kDa

UniProt: Q9Y2L1