CD84 antibody (AA 22-225)

Catalog No. ABIN6719602

This Rabbit Polyclonal antibody specifically detects CD84 in FACS, ELISA, IHC and ICC. It exhibits reactivity toward Human.

Quick Overview for CD84 antibody (AA 22-225) (ABIN6719602)

Target: CD84

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD84 antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-CD84 Antibody

Binding Specificity: AA 22-225

Purpose: Anti-CD84 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CD84 Antibody Picoband® (ABIN6719602). Tested in ELISA, Flow Cytometry, IHC, ICC applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human CD84 recombinant protein (Position: K22-G225).

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
Immunocytochemistry, 0.5-1 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5 μg/mL
1. "Entrez Gene: CD84 CD84 Molecule". 2. Tangye SG, Nichols KE, Hare NJ, van de Weerdt BC (Sep 2003). "Functional requirements for interactions between CD84 and Src homology 2 domain-containing proteins and their contribution to human T cell activation". Journal of Immunology. 171 (5): 2485-95.

Comment:

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CD84

Target: CD84

Alternative Name: CD84

Background:

Synonyms: SLAM family member 5, Cell surface antigen MAX.3, Hly9-beta, Leukocyte differentiation antigen CD84, Signaling lymphocytic activation molecule 5, CD84, SLAMF5

Tissue Specificity: Predominantly expressed in hematopoietic tissues, such as lymph node, spleen and peripheral leukocytes. Expressed in macrophages, B-cells, monocytes, platelets, thymocytes, T-cells and dendritic cells. Highly expressed in memory T-cells. Expressed in mast cells.

Background: CD84 (Cluster of Differentiation 84) is a human protein encoded by the CD84 gene. This gene encodes a membrane glycoprotein that is a member of the signaling lymphocyte activation molecule (SLAM) family. This family forms a subset of the larger CD2 cell-surface receptor Ig superfamily. The encoded protein is a homophilic adhesion molecule that is expressed in numerous immune cells types and is involved in regulating receptor-mediated signaling in those cells. Alternate splicing results in multiple transcript variants. Members of the CD2 subgroup of the Ig superfamily, such as CD84, have similar patterns of conserved disulfide bonds and function in adhesion interactions between T lymphocytes and accessory cells.

Molecular Weight: 19 kDa

Gene ID: 8832