PDE4D antibody (AA 466-709) (DyLight 550)

Catalog No. ABIN7354588

This anti-PDE4D antibody is a Rabbit Polyclonal antibody detecting PDE4D in FACS. Suitable for Human.

Quick Overview for PDE4D antibody (AA 466-709) (DyLight 550) (ABIN7354588)

Target: PDE4D (phosphodiesterase 4D, cAMP-Specific (PDE4D))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDE4D antibody is conjugated to DyLight 550

Application: Flow Cytometry (FACS)

Product Details anti-PDE4D Antibody (DyLight 550)

Binding Specificity: AA 466-709

Purpose: Anti-Human PDE4D DyLight® 550 conjugated Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Human PDE4D DyLight® 550 conjugated Antibody -Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human PDE4D recombinant protein (Position: H466-Q709).

Isotype: IgG

Application Details

Application Notes: Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells1. "Entrez Gene: PDE4D phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila)". 2. Michot C, Le Goff C, Goldenberg A, Abhyankar A, Klein C, Kinning E, Guerrot AM, Flahaut P, Duncombe A, Baujat G, Lyonnet S, Thalassinos C, Nitschke P, Casanova JL, Le Merrer M, Munnich A, Cormier-Daire V (Apr 2012). "Exome sequencing identifies PDE4D mutations as another cause of acrodysostosis". American Journal of Human Genetics. 90 (4): 740-5.

Comment:

Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Target Details for PDE4D

Target: PDE4D (phosphodiesterase 4D, cAMP-Specific (PDE4D))

Alternative Name: PDE4D

Background:

Synonyms: cAMP-specific 3',5'-cyclic phosphodiesterase 4D, DPDE3, PDE43, PDE4D, DPDE3

Tissue Specificity: Expressed in colonic epithelial cells (at protein level). Widespread, most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.

Background: cAMP-specific 3',5'-cyclic phosphodiesterase 4D is an enzyme that in humans is encoded by the PDE4D gene. This gene encodes one of four mammalian counterparts to the fruit fly 'dunce' gene. The encoded protein has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP, which acts as a signal transduction molecule in multiple cell types. This gene uses different promoters to generate multiple alternatively spliced transcript variants that encode functional proteins.

Molecular Weight: 39 kDa

Gene ID: 5144

UniProt: Q08499

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling