SCRIB antibody (AA 172-409) (DyLight 550)

Catalog No. ABIN7354662

This anti-SCRIB antibody is a Rabbit Polyclonal antibody detecting SCRIB in FACS. Suitable for Human.

Quick Overview for SCRIB antibody (AA 172-409) (DyLight 550) (ABIN7354662)

Target: SCRIB (Scribbled Homolog (SCRIB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SCRIB antibody is conjugated to DyLight 550

Application: Flow Cytometry (FACS)

Product Details anti-SCRIB Antibody (DyLight 550)

Binding Specificity: AA 172-409

Purpose: Anti-Human SCRIBBLE DyLight® 550 conjugated Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Human SCRIBBLE DyLight® 550 conjugated Antibody (ABIN5693286)-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human SCRIBBLE recombinant protein (Position: F172-K409).

Isotype: IgG

Application Details

Application Notes: Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells1. L E Dow, J S Kauffman, J Caddy, A S Peterson, S M Jane, S M Russell & P O Humbert (April 2007). "The tumour-suppressor Scribble dictates cell polarity during ed epithelial migration: regulation of Rho GTPase recruitment to the leading edge". Oncogene. 26 (16): 2272-82. 2. Yi Qin, Christopher Capaldo, Barry M. Gumbiner & Ian G. Macara (December 2005). "The mammalian SCRIB polarity protein regulates epithelial cell adhesion and migration through E-cadherin". JCB. 171 (6): 1061-71.

Comment:

Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Target Details for SCRIB

Target: SCRIB (Scribbled Homolog (SCRIB))

Alternative Name: SCRIB

Background:

Synonyms: Protein scribble homolog, Scribble, hScrib, Protein LAP4, SCRIB, CRIB1, KIAA0147, LAP4, SCRB1, VARTUL

Tissue Specificity: Expressed in kidney, skeletal muscles, liver, lung, breast, intestine, placenta and skin mainly in epithelial cells (at protein level).

Background: SCRIB, also known as Scribble, SCRIBL, or Scribbled homolog (Drosophila), is a scaffold protein which in humans is encoded by the SCRIB gene. In Drosophila melanogaster, SCRIB is involved in synaptic function, neuroblast differentiation, and epithelial polarization. Mechanistically, the human homolog is a scaffold protein linked to cellular differentiation centered on the regulation of epithelial as well as neuronal morphogenesis. Deficiency in SCRIB impairs many aspects of cell polarity and cell movement. SCRIB is also likely involved in establishing apical-basal polarity as well as progression from the G1 phase to S phase in the cell cycle as a result of its relationship with cell proliferation and exocytosis.

Molecular Weight: 39 kDa

Gene ID: 23513

UniProt: Q14160

Pathways: Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Tube Formation, Synaptic Vesicle Exocytosis, Asymmetric Protein Localization