RAB3GAP2 antibody (AA 1-115)

Catalog No. ABIN6719633

The Rabbit Polyclonal anti-RAB3GAP2 antibody has been validated for ELISA, WB and IHC. It is suitable to detect RAB3GAP2 in samples from Human, Mouse and Rat.

Quick Overview for RAB3GAP2 antibody (AA 1-115) (ABIN6719633)

Target: RAB3GAP2 (RAB3 GTPase Activating Protein Subunit 2 (Non-Catalytic) (RAB3GAP2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAB3GAP2 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-RAB3GAP2 Antibody

Binding Specificity: AA 1-115

Purpose: Anti-RAB3GAP2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RAB3GAP2 Antibody Picoband® (ABIN6719633). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RAB3GAP2 recombinant protein (Position: M1-Q115).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Borck, G., Wunram, H., Steiert, A., Volk, A. E., Korber, F., Roters, S., Herkenrath, P., Wollnik, B., Morris-Rosendahl, D. J., Kubisch, C. A homozygous RAB3GAP2 mutation causes Warburg Micro syndrome. Hum. Genet. 129: 45-50, 2011. 2. Handley, M. T., Morris-Rosendahl, D. J., Brown, S., Macdonald, F., Hardy, C., Bem, D., Carpanini, S. M., Borck, G., Martorell, L., Izzi, C., Faravelli, F., Accorsi, P., and 23 others. Mutation spectrum in RAB3GAP1, RAB3GAP2, and RAB18 and genotype-phenotype correlations in Warburg Micro syndrome and Martsolf syndrome. Hum. Mutat. 34: 686-696, 2013.

Comment:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for RAB3GAP2

Target: RAB3GAP2 (RAB3 GTPase Activating Protein Subunit 2 (Non-Catalytic) (RAB3GAP2))

Alternative Name: RAB3GAP2

Background:

Synonyms: Rab3 GTPase-activating protein non-catalytic subunit, RGAP-iso, Rab3 GTPase-activating protein 150 kDa subunit, Rab3-GAP p150, Rab3-GAP150, Rab3-GAP regulatory subunit, RAB3GAP2, KIAA0839

Tissue Specificity: Ubiquitous.

Background: Rab3 GTPase-activating protein non-catalytic subunit is an enzyme that in humans is encoded by the RAB3GAP2 gene. It is mapped to 1q41. The protein encoded by this gene belongs to the RAB3 protein family, members of which are involved in regulated exocytosis of neurotransmitters and hormones. This protein forms the Rab3 GTPase-activating complex with RAB3GAP1, where it constitutes the regulatory subunit, whereas the latter functions as the catalytic subunit. This gene has the highest level of expression in the brain, consistent with it having a key role in neurodevelopment. Mutations in this gene are associated with Martsolf syndrome.

Molecular Weight: 156 kDa

Gene ID: 25782

UniProt: Q9H2M9