CCDC36 antibody (AA 172-472)

Catalog No. ABIN6719645

The Rabbit Polyclonal anti-CCDC36 antibody has been validated for WB, ELISA, IHC, IF and ICC. It is suitable to detect CCDC36 in samples from Human.

Quick Overview for CCDC36 antibody (AA 172-472) (ABIN6719645)

Target: CCDC36 (Coiled-Coil Domain Containing 36 (CCDC36))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCDC36 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-CCDC36 Antibody

Binding Specificity: AA 172-472

Purpose: Anti-CCDC36 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CCDC36 Antibody Picoband® (ABIN6719645). Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CCDC36 recombinant protein (Position: E172-N472).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Stanzione M., Baumann M., Papanikos F., Dereli I., Lange J., Ramlal A., Traenkner D., Shibuya H., de Massy B., Watanabe Y., Jasin M., Keeney S., Toth A. Meiotic DNA break formation requires the unsynapsed chromosome axis-binding protein IHO1 (CCDC36) in mice. Nat. Cell Biol. 18:1208-1220(2016)

Comment:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CCDC36

Target: CCDC36 (Coiled-Coil Domain Containing 36 (CCDC36))

Alternative Name: CCDC36

Background:

Synonyms: Interactor of HORMAD1 protein 1, Cancer/testis antigen 74, CT74, Coiled-coil domain-containing protein 36, CCDC36, IHO1

Tissue Specificity: Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.

Background: CCDC36, also known as CT74 (cancer/testis antigen 74), is a 584 amino acid protein that contains coiled-coil domains and is expressed as three alternatively spliced isoforms. The gene encoding CCDC36 maps to human chromosome 3, which houses over 1,100 genes, including a chemokine receptor (CKR) gene cluster and a variety of human cancer-related gene loci. Key tumor suppressing genes on chromosome 3 include those that encode the apoptosis mediator RASSF1, the cell migration regulator HYAL1 and the angiogenesis suppressor SEMA3B. Marfan Syndrome, porphyria, von Hippel-Lindau syndrome, osteogenesis imperfecta and Charcot-Marie-Tooth Disease are a few of the numerous genetic diseases associated with chromosome 3.

Molecular Weight: 66 kDa

Gene ID: 339834