Mitochondrially Encoded NADH Dehydrogenase 4 (MT-ND4) (AA 10-459) antibody

Catalog No. ABIN6719648

The Rabbit Polyclonal anti- antibody has been validated for WB and ELISA. It is suitable to detect in samples from Human.

Quick Overview for Mitochondrially Encoded NADH Dehydrogenase 4 (MT-ND4) (AA 10-459) antibody (ABIN6719648)

Target: Mitochondrially Encoded NADH Dehydrogenase 4 (MT-ND4)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Product Details

Binding Specificity: AA 10-459

Purpose: Anti-MT-ND4 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MT-ND4 Antibody Picoband® (ABIN6719648). Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MT-ND4 recombinant protein (Position: M10-S459).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
ELISA, 0.1-0.5 μg/mL
1. "Entrez Gene: MT-ND4 mitochondrially encoded NADH dehydrogenase 4". 2. Valdivieso AG, Marcucci F, Taminelli G, Guerrico AG, Alvarez S, Teiber ML, Dankert MA, Santa-Coloma TA (May 2007). "The expression of the mitochondrial gene MT-ND4 is downregulated in cystic fibrosis".Biochemical and Biophysical Research Communications. 356 (3): 805-9.

Comment:

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details

Target: Mitochondrially Encoded NADH Dehydrogenase 4 (MT-ND4)

Alternative Name: MT-ND4

Background:

Synonyms: NADH-ubiquinone oxidoreductase chain 4, NADH dehydrogenase subunit 4, MT-ND4, MTND4, NADH4, ND4,

Tissue Specificity: Expressed in lung, on the vascular capillary network within alveolar walls, and also at lower level in kidney.

Background: NADH-ubiquinone oxidoreductase chain 4 is a protein that in humans is encoded by the mitochondrial gene MT-ND4. MT-ND4 is a subunit of the respiratory chain Complex I that is believed to belong to the minimal assembly of core proteins required to catalyze NADH dehydrogenation and electron transfer to ubiquinone (coenzyme Q10). Initially, NADH binds to Complex I and transfers two electrons to the isoalloxazine ring of the flavin mononucleotide (FMN) prosthetic arm to form FMNH2. The electrons are transferred through a series of iron-sulfur (Fe-S) clusters in the prosthetic arm and finally to coenzyme Q10 (CoQ), which is reduced to ubiquinol (CoQH2). The flow of electrons changes the redox state of the protein, resulting in a conformational change and pK shift of the ionizable side chain, which pumps four hydrogen ions out of the mitochondrial matrix.

Molecular Weight: 36 kDa

Gene ID: 4538

UniProt: P03905