CPAMD8 antibody (AA 58-234)

Catalog No. ABIN6719649

This anti-CPAMD8 antibody is a Rabbit Polyclonal antibody detecting CPAMD8 in ELISA, IHC and WB. Suitable for Human.

Quick Overview for CPAMD8 antibody (AA 58-234) (ABIN6719649)

Target: CPAMD8 (C3 and PZP-Like, alpha-2-Macroglobulin Domain Containing 8 (CPAMD8))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CPAMD8 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Product Details anti-CPAMD8 Antibody

Binding Specificity: AA 58-234

Purpose: Anti-CPAMD8 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CPAMD8 Antibody Picoband® (ABIN6719649). Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CPAMD8 recombinant protein (Position: R58-D234).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Cheong, S.-S., Hentschel, L., Davidson, A. E., Gerrelli, D., Davie, R., Rizzo, R., Pontikos, N., Plagnol, V., Moore, A. T., Sowden, J. C., Michaelides, M., Snead, M., Tuft, S. J., Hardcastle, A. J. Mutations in CPAMD8 cause a unique form of autosomal-recessive anterior segment dysgenesis. Am. J. Hum. Genet. 99: 1338-1352, 2016. 2. Li, Z.-F., Wu, X., Engvall, E. Identification and characterization of CPAMD8, a novel member of the complement 3/alpha-2-macroglobulin family with a C-terminal Kazal domain. Genomics 83: 1083-1093, 2004.

Comment:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CPAMD8

Target: CPAMD8 (C3 and PZP-Like, alpha-2-Macroglobulin Domain Containing 8 (CPAMD8))

Alternative Name: CPAMD8

Background:

Synonyms: C3 and PZP-like alpha-2-macroglobulin domain-containing protein 8, CPAMD8, KIAA1283

Tissue Specificity: Highly expressed in the kidney, brain and testis and to a lower extent in heart, liver and small intestine. Expressed in the lens, cornea and retina. Strongly expressed in the distal tips of the retinal neuroepithelium that form the iris and ciliary body.

Background: CPAMD8 belongs to the complement component-3 (C3, 120700)/alpha-2-macroglobulin (A2M, 103950) family of proteins, which are involved in innate immunity and damage control. The encoded protein is membrane-associated and proteolytically processed to generate two chains. Mutations in this gene cause a form of anterior segment dysgenesis, a developmental disorder of the eye. By genomic sequence analysis, the CPAMD8 gene is mapped to chromosome 19p13.3-p13.2.

Molecular Weight: 207 kDa

Gene ID: 27151