CD3 antibody (mFluor™450)

Catalog No. ABIN6731073

This anti-CD3 antibody is a Mouse Monoclonal antibody detecting CD3 in FACS. Suitable for Human.

Quick Overview for CD3 antibody (mFluor™450) (ABIN6731073)

Target: CD3

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 antibody is conjugated to mFluor™450

Application: Flow Cytometry (FACS)

Grade: GMP Grade

Clone: UCHT1

Product Details anti-CD3 Antibody (mFluor™450)

Purpose: CD3 mFluor™ 450 Antibody

Characteristics: The monoclonal UCHT1 clone recognizes CD3ε antigen, a 20KDa transmembrane cell-surface protein that belongs to the immunoglobulin superfamily. The CD3 complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains. These chains associate with a molecule known as the T-cell receptor (TCR) and the ζ-chain (zeta-chain) to generate an activation signal in T lymphocytes. CD3ε is expressed on T lymphocytes, NK-T cells, and to varying degrees on developing thymocytes. CD3 plays central roles in TCR signaling, T lymphocyte activation, and antigen recognition. Crosslinking of the TCR via plate bound UCHT1 monoclonal is widely used to study the activation T cell response in vitro.

Purification: Purified

Purity: >95 %

Immunogen: Thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS pH 7.2, 0.2 % (w/v) BSA, 0.09 % (w/v) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C, Conjugated antibodies should never be frozen.

Target Details for CD3

Target: CD3

Alternative Name: CD3

Gene ID: 916

NCBI Accession: NM_000733

UniProt: P07766

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway