FASL antibody (C-Term)

Catalog No. ABIN6735258

This anti-FASL antibody is a Rabbit Polyclonal antibody detecting FASL in WB and ELISA. Suitable for Human.

Quick Overview for FASL antibody (C-Term) (ABIN6735258)

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FASL antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-FASL Antibody

Binding Specificity: C-Term

Specificity: Sequence is 94 % conserved in mouse. It is poorly conserved in human FASL. 92 % homologous with rat and mouse FASL. Based upon the sequence homology data, anti human FASL should cross-react with the mouse/rat FASL.

Purification: Immunoaffinity purified

Immunogen: 13-aa peptide from human FASL near the C terminus.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), WB (1:1000 - 1:5000)

Usage: Suitable for use in ELISA and Western Blot. Western Blot: 1:1000-1:5000 (ECL). We recommend the use of 0.5-1 % milk in diluents used for primary antibody and secondary antibody-enzyme conjugates to suppress background. Rat, mouse and human FASL have 278, 279, and 281aa residues, respectively (Calculated MW of ~32kD). Western blot of the oocyte has detected a specific 31-kD band. A 40kD FASL has been detected in mouse T cells. An alternate detecting reagent, mFAS-Fc also detected a cell surface protein of about 40kD. ELISA: 0.5-1 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 100 mM Tris,  pH 7.5, 0.2 % BSA, 40 % glycerol.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for FASL

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Alternative Name: FASLG / FASL / Fas Ligand

Background: Name/Gene ID: FASLG
Family: TNF

Synonyms: FASLG, ALPS1B, Apoptosis antigen ligand, APTL, CD95L, CD95 ligand, CD178 antigen, CD95-L, Fas antigen ligand, Fas ligand, FASL, TNFSF6, APT1LG1, CD178

Gene ID: 356

UniProt: P48023

Pathways: Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity