SLC5A4 antibody (N-Term)

Catalog No. ABIN6735443

The Rabbit Polyclonal anti-SLC5A4 antibody has been validated for WB and ELISA. It is suitable to detect SLC5A4 in samples from Mouse.

Quick Overview for SLC5A4 antibody (N-Term) (ABIN6735443)

Target: SLC5A4 (Solute Carrier Family 5 (Low Affinity Glucose Cotransporter), Member 4 (SLC5A4))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC5A4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-SLC5A4 Antibody

Binding Specificity: N-Term

Specificity: Recognizes mouse SGLT-3. Mouse SG31-P is 100 % conserved in SGLT3-b (gene accession # Q9ET36, 657-660 aa) but not in mouse SLGLT-3-a isoforms (gene accession # Q9ET37, 656 aa) due to divergence at the n-terminus. No significant sequence homology exists with other SGLTs. Species sequence homology: human and porcine ~37 %.

Purification: Immunoaffinity purified

Immunogen: A 16 AA peptide sequence from the N-terminal, cytoplasmic domain 1 of Mouse SGLT-3 (1) was synthesized, coupled to KLH.

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:10000 - 1:50000), WB (1:1000 - 1:5000)

Usage: Suitable for use in ELISA, Western Blot. Western Blot: 1:1000-1:5000 for neat serum and 1-10 μg/mL for affinity pure using ECL. A band of 95-110kD has been detected in brush border membrane. ELISA:1:10000-1:50000 for neat serum and 0.5-1 μg/mL for affinity purified. Control peptide can be used to coat ELISA plates at 1 μg/mL.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4,1 % BSA

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for SLC5A4

Target: SLC5A4 (Solute Carrier Family 5 (Low Affinity Glucose Cotransporter), Member 4 (SLC5A4))

Alternative Name: SLC5A4 / SGLT2

Background: Name/Gene ID: SLC5A4
Subfamily: Solute:sodium symporter
Family: Transporter

Synonyms: SLC5A4, DJ90G24.4, Na(+)/glucose cotransporter 3, SAAT11, SGLT2, Sodium transporter, Sodium/glucose cotransporter 3, SAAT1, SGLT3

Gene ID: 6527

UniProt: Q9NY91

Pathways: Proton Transport