Adenosine A2b Receptor antibody (2nd Extracellular Domain)

Catalog No. ABIN6735512

This Rabbit Polyclonal antibody specifically detects Adenosine A2b Receptor in WB, ELISA, IHC, IF and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for Adenosine A2b Receptor antibody (2nd Extracellular Domain) (ABIN6735512)

Target: Adenosine A2b Receptor (ADORA2B)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Adenosine A2b Receptor antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Adenosine A2b Receptor Antibody

Binding Specificity: 2nd Extracellular Domain

Specificity: Recognizes Adenosine A2b Receptor. Species cross-reactivity: Mouse and rat A2bR. The peptide used demonstrates 75 % homology (12/16 AA) with rat, 62 % (11/16 AA) with mouse A2bR sequences.

Purification: Protein A purified

Immunogen: A 16 amino acid sequence corresponding to human brain A2bR gene (1). This region corresponds to the 2nd extracellular domain. A cysteine has been added at the NH2 terminus for coupling to KLH.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:10000 - 1:50000), IF (10 - 50 μg/mL), IHC, IHC-Fr, WB (1 - 10 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence. Western Blot: 1-10 μg/mL using ECL. ELISA: 1:10000-1:50000 using 50-100 ng A1R control peptide/well. Immunohistochemistry (frozen): 10-50 μg/mL. Immunofluorescence: 10-50 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.5, 0.1 % BSA.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for Adenosine A2b Receptor

Target: Adenosine A2b Receptor (ADORA2B)

Alternative Name: ADORA2B/Adenosine A2B Receptor

Background: Name/Gene ID: ADORA2B
Subfamily: Adenosine
Family: GPCR

Synonyms: ADORA2B, ADORA2, A2b receptor, Adenosine A2b receptor, Adenosine a2b, Adenosine receptor A2b, A2b adenosine receptor

Gene ID: 136

UniProt: P29275

Pathways: cAMP Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process