TRIM31 antibody (AA 117-166)

Catalog No. ABIN6736438

This anti-TRIM31 antibody is a Rabbit Polyclonal antibody detecting TRIM31 in WB, IHC and IHC (p). Suitable for Human, Cow and Monkey.

Quick Overview for TRIM31 antibody (AA 117-166) (ABIN6736438)

Target: TRIM31 (Tripartite Motif Containing 31 (TRIM31))

Reactivity: Human, Cow, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM31 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TRIM31 Antibody

Binding Specificity: AA 117-166

Brand: IHC-plus™

Specificity: Human TRIM31

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Rabbit (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa117-166 of human TRIM31 (Q9BZY9, NP_008959). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey (100%), Rabbit (92%), Mouse (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for TRIM31

Target: TRIM31 (Tripartite Motif Containing 31 (TRIM31))

Alternative Name: TRIM31 / RNF

Background: Name/Gene ID: TRIM31
Family: Tripartite Motif

Synonyms: TRIM31, C6orf13, HCGI, RNF, Tripartite motif-containing 31, HCG1, Ring finger protein, Tripartite motif containing 31

Gene ID: 11074

NCBI Accession: NP_008959

UniProt: Q9BZY9