IGF2BP1 antibody (AA 155-204)

Catalog No. ABIN6736731

The Rabbit Polyclonal anti-IGF2BP1 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect IGF2BP1 in samples from Human, Rat, Rabbit, Dog, Pig and Monkey.

Quick Overview for IGF2BP1 antibody (AA 155-204) (ABIN6736731)

Target: IGF2BP1 (Insulin-Like Growth Factor 2 mRNA Binding Protein 1 (IGF2BP1))

Reactivity: Human, Rat, Rabbit, Dog, Pig, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IGF2BP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-IGF2BP1 Antibody

Binding Specificity: AA 155-204

Brand: IHC-plus™

Specificity: Human IGF2BP1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Rat, Dog (100%) Bovine, Horse, Guinea pig (92%) Pig (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa155-204 of human IGF2BP1 (Q9NZI8, NP_006537). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Rat, Panda, Dog, Rabbit (100%), Mouse, Elephant, Bovine, Horse, Opossum, Guinea pig (92%), Turkey, Chicken (85%), Pig (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for IGF2BP1

Target: IGF2BP1 (Insulin-Like Growth Factor 2 mRNA Binding Protein 1 (IGF2BP1))

Alternative Name: CRD-BP / ZBP1 / IGF2BP1

Background: Name/Gene ID: IGF2BP1

Synonyms: IGF2BP1, CRD-BP, CRDBP, IMP1, IGF2 mRNA-binding protein 1, IMP-1, ZBP-1, Zip code-binding protein 1, Zipcode-binding protein 1, VICKZ family member 1, IGF-II mRNA-binding protein 1, VICKZ1

Gene ID: 10642

NCBI Accession: NP_006537

UniProt: Q9NZI8