ZFP36 antibody (AA 84-133)

Catalog No. ABIN214153

This Rabbit Polyclonal antibody specifically detects ZFP36 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Guinea Pig, Rabbit, Sheep and Pig.

Quick Overview for ZFP36 antibody (AA 84-133) (ABIN214153)

Target: ZFP36 (Zinc Finger Protein 36, C3H Type, Homolog (Mouse) (ZFP36))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Rabbit, Sheep, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZFP36 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ZFP36 Antibody

Binding Specificity: AA 84-133

Brand: IHC-plus™

Specificity: Human ZFP36

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Rat, Sheep, Dog, Bovine, Rabbit, Guinea pig (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa84-133 of human ZFP36 (P26651, NP_003398). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon (100%), Marmoset, Mouse, Rat, Sheep, Panda, Dog, Bovine, Rabbit, Guinea pig (85%), Elephant (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ZFP36

Target: ZFP36 (Zinc Finger Protein 36, C3H Type, Homolog (Mouse) (ZFP36))

Alternative Name: ZFP36 / Tristetraprolin

Background: Name/Gene ID: ZFP36

Synonyms: ZFP36, Protein TIS11A, Tristetraprolin, ZFP36 ring finger protein, TIS11, Zinc finger protein 36 homolog, TTP, NUP475, TIS11A, Tristetraproline, Zfp-36, G0S24, GOS24, RNF162A

Gene ID: 7538

NCBI Accession: NP_003398

UniProt: P26651