AIRE antibody (AA 218-267)

Catalog No. ABIN6737664

The Rabbit Polyclonal anti-AIRE antibody has been validated for WB. It is suitable to detect AIRE in samples from Human, Horse and Guinea Pig.

Quick Overview for AIRE antibody (AA 218-267) (ABIN6737664)

Target: AIRE (Autoimmune Regulator (AIRE))

Reactivity: Human, Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AIRE antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-AIRE Antibody

Binding Specificity: AA 218-267

Specificity: Human AIRE

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Horse, Guinea pig (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa218-267 of human AIRE (O43918, NP_000374). Percent identity by BLAST analysis: Human (100%), Gorilla, Horse, Guinea pig (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for AIRE

Target: AIRE (Autoimmune Regulator (AIRE))

Alternative Name: AIRE

Background: Name/Gene ID: AIRE

Synonyms: AIRE, APSI, AIRE1, Autoimmune regulator, APECED, APS1, APECED protein, PGA1

Gene ID: 326

NCBI Accession: NP_000374

UniProt: O43918

Pathways: Chromatin Binding