NHLH1 antibody (AA 38-87)

Catalog No. ABIN6739100

This anti-NHLH1 antibody is a Rabbit Polyclonal antibody detecting NHLH1 in WB. Suitable for Human.

Quick Overview for NHLH1 antibody (AA 38-87) (ABIN6739100)

Target: NHLH1 (Nescient Helix Loop Helix 1 (NHLH1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NHLH1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NHLH1 Antibody

Binding Specificity: AA 38-87

Specificity: Human NHLH1

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Bovine (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa38-87 of human NHLH1 (Q02575, NP_005589). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Marmoset (100%), Monkey, Bovine, Horse, Pig, Guinea pig (92%), Elephant, Opossum (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS, 2 % sucrose.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for NHLH1

Target: NHLH1 (Nescient Helix Loop Helix 1 (NHLH1))

Alternative Name: NHLH1 / NSCL

Background: Name/Gene ID: NHLH1

Synonyms: NHLH1, BHLHa35, Helix-loop-helix protein 1, HEN-1, HEN1, NSCL, Nescient helix loop helix 1, NSCL1, NSCL-1

Gene ID: 4807

NCBI Accession: NP_005589

UniProt: Q02575