PURA antibody (AA 35-84)

Catalog No. ABIN6740553

The Rabbit Polyclonal anti-PURA antibody has been validated for WB. It is suitable to detect PURA in samples from Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog, Rabbit, Sheep, Guinea Pig, Monkey and Pig.

Quick Overview for PURA antibody (AA 35-84) (ABIN6740553)

Target: PURA (Purine-Rich Element Binding Protein A (PURA))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog, Rabbit, Sheep, Guinea Pig, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PURA antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PURA Antibody

Binding Specificity: AA 35-84

Specificity: Human PURA

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Sheep, Dog, Bovine, Rabbit, Guinea pig, Zebrafish (100%) Xenopus (87%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa35-84 of human PURA (Q00577, NP_005850). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Panda, Dog, Bovine, Rabbit, Pig, Opossum, Guinea pig, Zebra finch, Zebrafish (100%), Xenopus (87%), Smelt (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PURA

Target: PURA (Purine-Rich Element Binding Protein A (PURA))

Alternative Name: PURALPHA / PURA

Background: Name/Gene ID: PURA

Synonyms: PURA, PUR-ALPHA, PURALPHA, PUR1

Gene ID: 5813

NCBI Accession: NP_005850

UniProt: Q00577