CD40 antibody (AA 35-84)

Catalog No. ABIN6740742

This Rabbit Polyclonal antibody specifically detects CD40 in WB. It exhibits reactivity toward Human, Rabbit, Monkey and Pig.

Quick Overview for CD40 antibody (AA 35-84) (ABIN6740742)

Target: CD40

Reactivity: Human, Rabbit, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD40 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CD40 Antibody

Binding Specificity: AA 35-84

Specificity: Human CD40

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Guinea pig (90%) Pig (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa35-84 of human CD40 (P25942, NP_690593). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Marmoset, Rabbit (100%), Galago, Guinea pig (90%), Horse, Pig (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for CD40

Target: CD40

Alternative Name: CD40

Background: Name/Gene ID: CD40
Family: TNF Receptor

Synonyms: CD40, B-cell surface antigen CD40, B cell-associated molecule, B cell surface antigen CD40, CD40 antigen, CD40 type II isoform, p50, Bp50, CD40L receptor, CDW40, TNFRSF5

Gene ID: 958

NCBI Accession: NP_690593

UniProt: P25942

Pathways: NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints