NFIX antibody (AA 181-230)

Catalog No. ABIN6741545

This anti-NFIX antibody is a Rabbit Polyclonal antibody detecting NFIX in WB, IHC and IHC (p). Suitable for Human, Rat, Mouse, Dog, Cow, Guinea Pig, Horse, Rabbit, Bat, Hamster, Monkey and Pig.

Quick Overview for NFIX antibody (AA 181-230) (ABIN6741545)

Target: NFIX (MLZE) (Nuclear Factor I/X (MLZE))

Reactivity: Human, Rat, Mouse, Dog, Cow, Guinea Pig, Horse, Rabbit, Bat, Hamster, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFIX antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NFIX Antibody

Binding Specificity: AA 181-230

Brand: IHC-plus™

Specificity: Human NFIX

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (100%) Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa181-230 of human NFIX (Q14938-3, NP_002492). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Stickleback (100%), Lizard, Zebrafish (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for NFIX

Target: NFIX (MLZE) (Nuclear Factor I/X (MLZE))

Alternative Name: NF1A / NFIX

Background: Name/Gene ID: NFIX

Synonyms: NFIX, CTF, NF1A, NFI-X, NF-I/X, Nuclear factor I/X, TGGCA-binding protein, Nuclear factor 1/X, MRSHSS, NF1-X, Nuclear factor 1 X-type, SOTOS2

Gene ID: 4784

NCBI Accession: NP_002492

UniProt: Q14938

Pathways: Skeletal Muscle Fiber Development