Sp3 antibody (AA 287-336)

Catalog No. ABIN6741550

The Rabbit Polyclonal anti-Sp3 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect Sp3 in samples from Human, Mouse, Rat, Guinea Pig, Horse, Rabbit, Cow, Dog, Bat, Monkey and Pig.

Quick Overview for Sp3 antibody (AA 287-336) (ABIN6741550)

Target: Sp3 (Sp3 Transcription Factor (Sp3))

Reactivity: Human, Mouse, Rat, Guinea Pig, Horse, Rabbit, Cow, Dog, Bat, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Sp3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Sp3 Antibody

Binding Specificity: AA 287-336

Brand: IHC-plus™

Specificity: Human SP3

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig (100%) Chicken (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa287-336 of human SP3 (Q02447, NP_003102). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Opossum, Chicken (92%), Turkey, Zebra finch, Platypus (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Sp3

Target: Sp3 (Sp3 Transcription Factor (Sp3))

Alternative Name: SP3

Background: Name/Gene ID: SP3

Synonyms: SP3, SPR2, Transcription factor Sp3, Sp3 transcription factor, SPR-2, Specificity protein 3

Gene ID: 6670

NCBI Accession: NP_003102

UniProt: Q02447