TFAP2C antibody (AA 252-301)

Catalog No. ABIN6741554

This anti-TFAP2C antibody is a Rabbit Polyclonal antibody detecting TFAP2C in WB, IHC and IHC (p). Suitable for Human, Mouse, Rat, Dog, Cow, Zebrafish (Danio rerio), Monkey, Rabbit, Pig, Guinea Pig, Horse, Sheep, Chicken, Xenopus laevis and Bat.

Quick Overview for TFAP2C antibody (AA 252-301) (ABIN6741554)

Target: TFAP2C (Transcription Factor AP-2 gamma (Activating Enhancer Binding Protein 2 Gamma) (TFAP2C))

Reactivity: Human, Mouse, Rat, Dog, Cow, Zebrafish (Danio rerio), Monkey, Rabbit, Pig, Guinea Pig, Horse, Sheep, Chicken, Xenopus laevis, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TFAP2C antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TFAP2C Antibody

Binding Specificity: AA 252-301

Brand: IHC-plus™

Specificity: Human TFAP2C / AP2 Gamma

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Chicken, Xenopus, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa252-301 of human TFAP2C (Q92754, NP_003213). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Seabass, Salmon, Stickleback, Zebrafish (100%), Panda, Lizard, Drosophila (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for TFAP2C

Target: TFAP2C (Transcription Factor AP-2 gamma (Activating Enhancer Binding Protein 2 Gamma) (TFAP2C))

Alternative Name: TFAP2C / AP2 Gamma

Background: Name/Gene ID: TFAP2C

Synonyms: TFAP2C, AP2 Gamma, AP-2 gamma, ERF-1, ERF1, HAP-2g, Transcription factor ERF-1, TFAP2G, AP2-GAMMA, Estrogen receptor factor 1

Gene ID: 7022

NCBI Accession: NP_003213

UniProt: Q92754

Pathways: Stem Cell Maintenance