TEAD1 antibody (C-Term)

Catalog No. ABIN462220

This Rabbit Polyclonal antibody specifically detects TEAD1 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Zebrafish (Danio rerio), Rabbit, Guinea Pig, Horse, Pig, Chicken, Monkey, Bat and Xenopus laevis.

Quick Overview for TEAD1 antibody (C-Term) (ABIN462220)

Target: TEAD1 (TEA Domain Family Member 1 (SV40 Transcriptional Enhancer Factor) (TEAD1))

Reactivity: Human, Mouse, Rat, Dog, Cow, Zebrafish (Danio rerio), Rabbit, Guinea Pig, Horse, Pig, Chicken, Monkey, Bat, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TEAD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TEAD1 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Human TEAD1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Guinea pig, Chicken, Xenopus, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human TEAD1 (P28347, NP_068780). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Xenopus, Zebrafish (100%), Panda, Platypus, Stickleback (92%), Lizard (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 - 10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for TEAD1

Target: TEAD1 (TEA Domain Family Member 1 (SV40 Transcriptional Enhancer Factor) (TEAD1))

Alternative Name: TEAD1

Background: Name/Gene ID: TEAD1

Synonyms: TEAD1, AA, Protein GT-IIC, TCF-13, TEAD-1, TEF-1, TEF1, TCF13, TEA domain family member 1, Transcription factor 13, NTEF-1, REF1

Gene ID: 7003

NCBI Accession: NP_068780

UniProt: P28347

Pathways: Regulation of Lipid Metabolism by PPARalpha